Carboxyl retained hapten of quinclorac produces highly sensitive antibody and immunochromatographic assay

文献类型： 外文期刊

第一作者： Ding, Yuan

作者： Ding, Yuan;Huang, Yue;Sun, Wanlin;Li, Fangxuan;Wang, Minghua;Hua, Xiude;Zhang, Shuguang;Fang, Song

作者机构：

关键词： Quinclorac; Hapten; Immunoassay; Monoclonal antibody; Immunochromatographic assay

期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：4.8； 五年影响因子：4.5 ）

ISSN： 0026-265X

年卷期： 2022 年 183 卷

页码：

收录情况： SCI

摘要： In order to obtain better anti-quinclorac antibody, we synthesized three novel quinclorac haptens de novo, including a hapten (H3) retains the carboxyl. As expected, H3 immunized mice showed the best immune re-sponses and generated the anti-quinclorac monoclonal antibody (mAb 6D2F4) with the 50 % inhibition con-centration (IC50) of 1.63 ng mL-1 in indirect competitive enzyme-linked immunosorbent assay, which is improved 30-to 245-fold than the reported anti-quinclorac antibodies, and no significant cross-reactivities with the analogues of quinclorac. Then mAb 6D2F4 was employed to develop the colloidal gold immunochromato-graphic assay (GICA) for the detection of quinclorac. The limit of detection (LOD) of naked eye observation is 8 ng mL-1, while the LODs of GICA in agro-products samples also can meet the requirements regulated by China. Besides, the detection results of blind samples by GICA show high consistency with those by high performance liquid chromatography analysis.

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