Plasmonic colorimetry and G-quadruplex fluorescence-based aptasensor: A dual-mode, protein-free and label-free detection for OTA

文献类型： 外文期刊

第一作者： Li, Ming

作者： Li, Ming;Qiu, Youxin;Liu, Guoxing;Xiao, Yu;Tian, Ye;Xiao, Yu;Fang, Song;Li, Ming

作者机构：

关键词： Ochratoxin A; Surface plasmon resonance; G -quadruplex; Gold nanoparticle; Dual -mode detection

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2024 年 448 卷

页码：

收录情况： SCI

摘要： G-quadruplexes (G4) have received significant attention in the field of aptasensors owing to their unique physicochemical characteristics. A dual-mode, protein-free and label-free aptamer sensor based on plasmonic colorimetry and G4 fluorescence (PC@GF-aptasensor) was proposed for ochratoxin A (OTA). Colorimetry mode was achieved through the surface plasmon resonance (SPR) effect, which related to the OTA-Apt-based G4-OTA. The fluorescence mode was reflected by the insertion of thioflavin T (ThT) into G4-OTA. The OTA could be interpreted via three readouts: (1) naked eye (LOD of 2.0 ng mL-1), (2) smartphone (LOD of 1.65 ng mL-1), and (3) spectrofluorometer (LOD of 0.93 ng mL-1). The PC@GF-aptasensor exhibited several advantages, such as a standardised recognition group, simplified operation, low background signal, and practicality. The proposed PC@GF-aptasensor integrated SPR-based multicolour interpretation and ThT-inserted fluorescence reflection to obtain a dual-mode optical biosensor, which may provide valuable insights for the development of other targets with G4-based aptamers.

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