Viral metagenomic analysis reveals diverse viruses and a novel bocaparvovirus in the enteric virome of snow leopard ( Panthera uncia) )

文献类型： 外文期刊

第一作者： Chukwudozie, Kingsley Ikechukwu

作者： Chukwudozie, Kingsley Ikechukwu;Lu, Chunying;Xue, Jiaxin;Zhang, Wen;Chukwudozie, Kingsley Ikechukwu;Wang, Haoning;Wang, Haoning;Wang, Xiaolong;Shan, Tongling

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关键词： Bocarparvovirus; Virome; Snow leopard; Viruses; Metagenomics

期刊名称：HELIYON （ 影响因子：3.4； 五年影响因子：3.9 ）

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年卷期： 2024 年 10 卷 8 期

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收录情况： SCI

摘要： The enteric virome, comprising a complex community of viruses inhabiting the gastrointestinal tract, plays a significant role in health and disease dynamics. In this study, the fecal sample of a wild snow leopard was subjected to viral metagenomic analysis using a double barcode Illumina MiSeq platform. The resulting reads were de novo assembled into contigs with SOAPdenovo2 version r240. Additional bioinformatic analysis of the assembled genome and genome annotation was done using the Geneious prime software (version 2022.0.2). Following viral metagenomic analysis and bioinformatic analysis, a total of 7 viral families and a novel specie of bocaparvovirus tentatively named Panthera uncia bocaparvovirus (PuBOV) with GenBank accession number OQ627713 were identified. The complete genome of PuBOV was predicted to contain 3 open reading frames (ORFs), contains 5433 nucleotides and has a G + C content of 47.40 %. BLASTx analysis and pairwise sequence comparison indicated the novel virus genome was a new species in the genus Bocaparvovirus based on the species demarcation criteria of the International Committee on the Taxonomy of Viruses. This study provides valuable insights into the diversity and composition of the enteric virome in wild endangered snow leopards. The identification and characterization of viruses in wildlife is crucial for developing effective strategies to manage and mitigate potential zoonotic and other viral disease threats to human and animal health.

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