Reverse design of haptens based on antigen spatial conformation to prepare anti-capsaicinoids&gingerols antibodies for monitoring of gutter cooking oil
文献类型: 外文期刊
第一作者: Nie, Kunying
作者: Nie, Kunying;Zhang, Jiali;Xu, Haitao;Ren, Keyun;Yu, Chunlei;Li, Falan;Yang, Qingqing;Zhang, Qi;Nie, Kunying;Zhang, Jiali;Xu, Haitao;Ren, Keyun;Yu, Chunlei;Li, Falan;Yang, Qingqing;Nie, Kunying;Zhang, Jiali;Xu, Haitao;Ren, Keyun;Yu, Chunlei;Li, Falan;Yang, Qingqing;Zhang, Qi;Zhang, Qi
作者机构:
关键词: Gutter cooking oil; Broad-spectrum monoclonal antibodies; Molecular docking; Virtual screening
期刊名称:FOOD CHEMISTRY-X ( 影响因子:6.1; 五年影响因子:6.4 )
ISSN: 2590-1575
年卷期: 2024 年 22 卷
页码:
收录情况: SCI
摘要: Rapid simultaneous detection of multi -component adulteration markers can improve the accuracy of identification of gutter cooking oil in edible oil, which is made possible by broad-spectrum antibody (bs-mAb). This study used capsaicinoids (CPCs) and gingerol derivatives (GDs) as adulteration markers, and two broad-spectrum haptens (bs-haptens) were designed and synthesized based on a reverse design strategy of molecular docking. Electrostatic potential (ESP) and monoclonal antibodies (mAbs) preparation verified the strategy ' s feasibility. To further investigate the recognition mechanism, five other reported antigens and mAbs were also used. Finally, the optimal combination (Hapten 5-OVA/1-F12) and key functional groups (f -groups) were determined. The half maximal inhibitory concentration (IC 50 ) for CPCs-GDs was between 88.13 and 499.16 ng/mL. Meanwhile, a preliminary lateral flow immunoassay (LFIA) study made practical monitoring possible. The study provided a theoretical basis for the virtual screening of bs-haptens and simultaneous immunoassay of multiple exogenous markers to monitor gutter oil rapidly and accurately.
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