Reverse design of haptens based on antigen spatial conformation to prepare anti-capsaicinoids&gingerols antibodies for monitoring of gutter cooking oil

文献类型： 外文期刊

第一作者： Nie, Kunying

作者： Nie, Kunying;Zhang, Jiali;Xu, Haitao;Ren, Keyun;Yu, Chunlei;Li, Falan;Yang, Qingqing;Zhang, Qi;Nie, Kunying;Zhang, Jiali;Xu, Haitao;Ren, Keyun;Yu, Chunlei;Li, Falan;Yang, Qingqing;Nie, Kunying;Zhang, Jiali;Xu, Haitao;Ren, Keyun;Yu, Chunlei;Li, Falan;Yang, Qingqing;Zhang, Qi;Zhang, Qi

作者机构：

关键词： Gutter cooking oil; Broad-spectrum monoclonal antibodies; Molecular docking; Virtual screening

期刊名称：FOOD CHEMISTRY-X （ 影响因子：6.1； 五年影响因子：6.4 ）

ISSN： 2590-1575

年卷期： 2024 年 22 卷

页码：

收录情况： SCI

摘要： Rapid simultaneous detection of multi -component adulteration markers can improve the accuracy of identification of gutter cooking oil in edible oil, which is made possible by broad-spectrum antibody (bs-mAb). This study used capsaicinoids (CPCs) and gingerol derivatives (GDs) as adulteration markers, and two broad-spectrum haptens (bs-haptens) were designed and synthesized based on a reverse design strategy of molecular docking. Electrostatic potential (ESP) and monoclonal antibodies (mAbs) preparation verified the strategy ' s feasibility. To further investigate the recognition mechanism, five other reported antigens and mAbs were also used. Finally, the optimal combination (Hapten 5-OVA/1-F12) and key functional groups (f -groups) were determined. The half maximal inhibitory concentration (IC 50 ) for CPCs-GDs was between 88.13 and 499.16 ng/mL. Meanwhile, a preliminary lateral flow immunoassay (LFIA) study made practical monitoring possible. The study provided a theoretical basis for the virtual screening of bs-haptens and simultaneous immunoassay of multiple exogenous markers to monitor gutter oil rapidly and accurately.

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