Construction of transgenic Bacillus mucilaginosus strain with improved phytase secretion
文献类型: 外文期刊
第一作者: Li, X
作者: Li, X;Yang, SH;Yu, XC;Jin, ZX;Li, WD;Li, L;Li, J;Li, MG
作者机构:
关键词: Bacillus mucilaginosus;microbial fertilizer;phytase;Tn5 transposition;ASPERGILLUS-NIGER PHYTASE;FUNGAL PHYA GENE;ORGANIC PHOSPHORUS;STABLE PHYTASE;EXPRESSION;SUBTILIS;TRANSPOSON;PHOSPHATE;SEEDLINGS;TOBACCO
期刊名称:JOURNAL OF APPLIED MICROBIOLOGY ( 影响因子:3.772; 五年影响因子:3.963 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Aims: To construct a transgenic Bacillus mucilaginosus strain to increase the secretion capability of a wild-type isolate of B. mucilaginosus D4B1 to hydrolyse phytate phosphorus, which can be used as a microbial fertilizer in field application.Methods and Results: We constructed a phytase secreting expression vector pSP43 with a mini-Tn5 transposon and a Aspergillus fumigatus phytase expression cassette. The vector pSP43 was successfully transferred into the wild-type B. mucilaginosus using the particle bombardment method, and three transgenic strains with a stable copy of phytase expression cassette integrated into the chromosome of the B. mucilaginosus by Tn5 transposition were selected. The phytase activity of the engineered strains increased 36-46-fold when compared with the wild-type strain of D4B1.Conclusions: The A. fumigatus phytase gene can be expressed under the direction of p43 promoter in B. mucilaginosus. The expression protein is secreted extracellularly and newly constructed strains showed a high phytase activity.Significance and Impact of the Study: A transgenic Bacillus strain by the particle bombardment method was constructed.
分类号: Q93
- 相关文献
作者其他论文 更多>>
-
Genetic diversity in three Japanese flounder (Paralichthys olivaceus) populations revealed by ISSR markers
作者:Liu, YG;Chen, SL;Li, J;Li, BF
关键词:Japanese flounder;Paralichthys olivaceus;ISSR marker;genetic diversity;genetic variation
-
Protein characteristics of Chinese black-grained wheat
作者:Li, WD;Beta, T;Sun, SC;Corke, H
关键词:wheat;black wheat;protein;digestibility;gluten index;mixing properties;amino acid composition;electrophoresis
-
Using Cs-137 and Pb-210(ex) for quantifying soil organic carbon redistribution affected by intensive tillage on steep slopes
作者:Li, Y;Zhang, QW;Reicosky, DC;Bai, LY;Lindstrom, MJ;Li, L
关键词:soil organic carbon (SOC);(137)cesium (Cs-137);excess Pb-210 (Pb-210);tillage erosion
-
Mapping of a wheat resistance gene to yellow mosaic disease by amplified fragment length polymorphism and simple sequence repeat markers
作者:Nie, H;He, ZT;Chen, XL;Han, YP;Wang, JR;Li, X;Han, CG;Yu, JL
关键词:locus on chromosome;molecular marker;resistance;simple sequence repeat;wheat;yellow mosaic disease
-
Photoprotective effects of high level expression Of C(4) phosphoenolpyruvate carboxylase in transgenic rice during photoinhibition
作者:Jiao, DM;Li, X;Ji, BH
关键词:carbon assimilation;carbonic anhydrase;chlorophyll fluorescence;3,3-dichloro-2-(dihydroxyphosphinoylmethyl)propenoate;enzymes scavenging active oxygen;malonyldialdehyde;Oryza;peroxidase;superoxide dismutase
-
Mapping a resistance gene in wheat cultivar Yangfu 9311 to yellow mosaic virus, using microsatellite markers
作者:Liu, WH;Nie, H;Wang, SB;Li, X;He, ZT;Han, CG;Wang, JR;Chen, XL;Li, LH;Yu, JL
关键词:chromosome localization;SSR marker;wheat;yellow mosaic disease
-
Modeling of waterflow and nitrate transport under surface drip fertigation
作者:Li, J;Zhang, J;Rao, M
关键词:drip irrigation;fertigation;nitrate;simulation;transport;water