Construction of transgenic Bacillus mucilaginosus strain with improved phytase secretion

文献类型： 外文期刊

第一作者： Li, X

作者： Li, X;Yang, SH;Yu, XC;Jin, ZX;Li, WD;Li, L;Li, J;Li, MG

作者机构：

关键词： Bacillus mucilaginosus;microbial fertilizer;phytase;Tn5 transposition;ASPERGILLUS-NIGER PHYTASE;FUNGAL PHYA GENE;ORGANIC PHOSPHORUS;STABLE PHYTASE;EXPRESSION;SUBTILIS;TRANSPOSON;PHOSPHATE;SEEDLINGS;TOBACCO

期刊名称：JOURNAL OF APPLIED MICROBIOLOGY （ 影响因子：3.772； 五年影响因子：3.963 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Aims: To construct a transgenic Bacillus mucilaginosus strain to increase the secretion capability of a wild-type isolate of B. mucilaginosus D4B1 to hydrolyse phytate phosphorus, which can be used as a microbial fertilizer in field application.Methods and Results: We constructed a phytase secreting expression vector pSP43 with a mini-Tn5 transposon and a Aspergillus fumigatus phytase expression cassette. The vector pSP43 was successfully transferred into the wild-type B. mucilaginosus using the particle bombardment method, and three transgenic strains with a stable copy of phytase expression cassette integrated into the chromosome of the B. mucilaginosus by Tn5 transposition were selected. The phytase activity of the engineered strains increased 36-46-fold when compared with the wild-type strain of D4B1.Conclusions: The A. fumigatus phytase gene can be expressed under the direction of p43 promoter in B. mucilaginosus. The expression protein is secreted extracellularly and newly constructed strains showed a high phytase activity.Significance and Impact of the Study: A transgenic Bacillus strain by the particle bombardment method was constructed.

分类号： Q93

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