Sequence variation of Bemisia tabaci Chemosensory Protein 2 in cryptic species B and Q: New DNA markers for whitefly recognition

文献类型： 外文期刊

第一作者： Liu, Guo-xia

作者： Liu, Guo-xia;Ma, Hong-mei;Xie, Hong-yan;Xuan, Ning;Picimbon, Jean-Francois;Picimbon, Jean-Francois;Picimbon, Jean-Francois

作者机构：

关键词： Bemisia tabaci; chemosensory protein; BtabCSP2; Intron; Evolution; cleaved amplified polymorphic sequences

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2016 年 576 卷 1 期

页码：

收录情况： SCI

摘要： Bemisia tabaci Gennadius biotypes B and Qare two of the most important worldwide agricultural insect pests. Genomic sequences of Type-2 B. tabaci chemosensory protein (BtabCSP2) were cloned and sequenced in B and Q biotypes, revealing key biotype-specific variations in the intron sequence. A Q260 sequence was found specifically in Q-BtabCSP2 and Cucumis melo LN692399, suggesting ancestral horizontal transfer of gene between the insect and the plant through bacteria. A cleaved amplified polymorphic sequences (CAPS) method was then developed to differentiate B and Qbased on the sequence variation in exon of BtabCSP2 gene. The performances of CSP2-based CAPS for whitefly recognition were assessed using B. tabaci field collections from Shandong Province (P.R. China). Our Sadl based CAPS method led to the same result compared to mitochondrial cytochrome oxidase-based CAPS method in the field collections. We therefore propose an explanation for CSP origin and a new rapid simple molecular method based on genomic DNA and chemosensory gene to differentiate accurately the B and Qwhiteflies of the Bemisia complex around the world. (C) 2015 Elsevier B.V. All rights reserved.

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