A multiple-population study reveals genetic variation and genes associated with resistance to Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus)

文献类型： 外文期刊

第一作者： Wang, Zhang

作者： Wang, Zhang;Liu, Zhigang;Huang, Shaowei;Zhu, Weijuan;Cao, Jianmeng;Wang, Miao;Yi, Mengmeng;Lu, Maixin;Ke, Xiaoli;Liu, Zhigang;Huang, Shaowei;Zhu, Weijuan;Lu, Maixin;Ke, Xiaoli

作者机构：

关键词： Tilapia; Streptococcus agalactiae; GWAS; Selective sweep analysis; SNP marker

期刊名称：AQUACULTURE （ 影响因子：3.9； 五年影响因子：4.4 ）

ISSN： 0044-8486

年卷期： 2026 年 611 卷

页码：

收录情况： SCI

摘要： Streptococcosis caused by Streptococcus agalactiae (S. agalactiae) is one of the most severe diseases of tilapia. However, the resistance mechanisms and molecular markers of this disease are still unclear. Herein, by using genome-wide association studies (GWAS) and selective sweep analysis, SNPs and genes associated with streptococcosis resistance were identified from six commercial Nile tilapia (Oreochromis niloticus) populations. A challenged test showed that these populations exhibited varying resistance to streptococcosis. The results indicated that four ancestral lineages contributed to four well-differentiated clades, with one clade existing at a higher frequency in susceptible individuals in each population (K = 4). A total of 214 SNPs were associated with the survival time of tilapia via single- and multi-population GWAS after challenge with S. agalactiae. These SNPs were widely distributed across 22 chromosomes, with 20 located in selective sweep regions. After validation using re-sequencing data and Kompetitive Allele Specific PCR (KASP) analysis, the GG genotype of LG11_20,310,698 significantly improved survival time compared to the other two genotypes. The frequency of the GG genotype was significantly higher in populations with lower mortality rates (P1, P2, P4, P5) than in the populations with the highest mortality rate (P3). LG11_20,310,698 could be annotated to eight immune-related genes, including five major histocompatibility complex (MHC) class II molecules, multi-drug resistance protein 1like, and NMT2. Notably, LG11_20,310,698 resulted in a missense mutation in ABCB4. Together, this study not only provides novel insights into the population structure of commercial tilapia populations in China but also explores effective SNPs and candidate genes for improving streptococcosis resistance in tilapia breeding.

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