A RUBY Reporter for Efficient Banana Transformation and Development of Betalain-Rich Musa Germplasm
文献类型： 外文期刊
第一作者： He, Weidi
作者： He, Weidi;Huang, Huoqing;Wang, Shuxian;Wang, Dalin;Xie, Yanling;Hu, Chunhua;Wang, Shuxian
作者机构：
关键词： banana; betalain; genetic transformation; RUBY
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）
ISSN： 1661-6596
年卷期： 2025 年 26 卷 16 期
页码：
收录情况： SCI
摘要： Bananas are economically important crops valued for both their nutritional and dietary uses. However, the global banana industry suffers from a narrow base dominated by a single variety. Developing novel varieties enriched in health-promoting compounds such as betalains can help diversify banana germplasm and meet evolving consumer demands. In this study, the RUBY reporter system was employed to produce betalain-rich bananas via stable and transient genetic transformations. Transient transformation by injecting 3 mL of Agrobacterium suspension into immature fruits produced vivid red-purple pulp containing up to 1.78 mg/g of betalains. For stable transformation, embryonic cell suspensions expressing RUBY exhibited a red-purple coloration after the first screening, reducing the selection period from 45 to 15 days. These findings demonstrate that RUBY is a reliable visual reporter for efficient screening and can be used to develop nutritionally enhanced bananas.
分类号：
- 相关文献
作者其他论文 更多>>
-
Alleviating Clostridium perfringens-Induced Intestinal Lesions in Chickens Using the Xylanase CbXyn10C and Its Binary Cocktail with a Protease
作者：Zhang, Wenjing;Hao, Zhenzhen;Yang, Daoxin;Ji, Wangli;Guo, Kairui;Sun, Xianhua;Wang, Shuai;Yang, Shuyan;Ma, Jianshuang;Luo, Huiying;Yao, Bin;Wang, Yuan;Huang, Huoqing;Su, Xiaoyun;Wang, Tong;Zhang, Meiling
关键词：necrotic enteritis; Clostridium perfringens; xylanase; protease; broiler chickens; gut microbiota modulation
-
Fructose metabolism in Entner-Doudoroff pathway-deficient Cupriavidus necator H16 depends on the Calvin shunt
作者：Ding, Lijuan;Guo, Gang;Cui, Lin;Wang, Yuheng;Liu, Xu;Luo, Huiying;Huang, Huoqing;Su, Xiaoyun;Bai, Yingguo;Zhang, Jie;Tu, Tao;Qin, Xing;Wang, Yuan;Wang, Yaru;Yao, Bin;Wang, Xiaolu;Dronsella, Beau;Xue, Xianli
关键词：Cupriavidus necator; Entner-Doudoroff pathway; Calvin shunt; Phosphoglycolate salvage pathway
-
Precise A
T-to-G C Base Editing in Banana 作者：Wu, Shaoping;Wu, Shaoping;Hu, Chunhua;Dou, Tongxin;Qin, Fei;Yin, Binhui;Bi, Fangcheng;Yang, Qiaosong;Yi, Ganjun;Hu, Chunhua;Dou, Tongxin;Bi, Fangcheng;Yang, Qiaosong;Yi, Ganjun;Zhu, Haocheng;Liu, Jinxing;Zhu, Haocheng;Yin, Binhui
关键词：Banana; Adenine base editor;
MaPDS ; Paraffin section; Deep amplicon sequencing -
Fusaric acid-mediated S-glutathionylation of MaAKT1 channel confers the virulence of Foc TR4 to banana
作者：Zhang, Jun;Zhang, Zhi-Ren;Zhang, Jun;Yao, Xiaoqiang;Liu, Siwen;Yang, Wenlong;Xie, Yanling;Shao, Chuange;Li, Chunyu;Liu, Siwen;Li, Chunyu;Liu, Siwen;Li, Chunyu;Yao, Xiaoqiang
关键词：
-
Metabolic Engineering of Escherichia coli for Efficient
l -Isoleucine Production based on the Citramalate Pathway作者：Zhang, Qiquan;Wang, Yuheng;Wang, Xiaolu;Bai, Yingguo;Wang, Yaru;Wang, Yuan;Tu, Tao;Qin, Xing;Su, Xiaoyun;Yao, Bin;Luo, Huiying;Liu, Xu;Huang, Huoqing;Zhang, Jie
关键词：Escherichia coli; l-isoleucine; metabolic engineering; citramalate pathway; threonine-independent pathway
-
Identification of Aspartate Aminotransferase FocAST2 as a Novel Target of Albendazole in Fusarium oxysporum f. sp. cubense TR4
作者：Liu, Yushan;Liu, Siwen;Peng, Cheng;Huang, Huoqing;Zhang, Wei;Huo, Yile;Yi, Ganjun;Li, Chunyu;Zeng, Weiqing
关键词：banana;
Foc TR4; albendazole; target; aspartate transaminase -
Genome-Wide Profiling of bZIP Transcription Factors and FocbZIP11's Impact on Fusarium TR4 Pathogenicity
作者：Xie, Yanling;Huang, Huoqing;Huo, Yile;Yang, Wenlong;Li, Yuqing;Liu, Siwen;Li, Chunyu;Xie, Yanling;Xie, Yanling;Liu, Siwen;Li, Chunyu;Liu, Siwen;Li, Chunyu
关键词：
Fusarium oxysporum f. sp.cubense tropical race 4; fungal stress adaptation; plant-pathogen interactions; virulence
