Isolation and characterization of the mink interferon-epsilon gene and its antiviral activity

文献类型： 外文期刊

第一作者： Zhang, Hailing

作者： Zhang, Hailing;Zhang, Dongliang;Hu, Bo;Lian, Shizhen;Bai, Xue;Zhang, Hailing;Zhang, Shasha;Wang, Han;Wang, Cong;Zou, Deying;Lu, Shiying;Liu, Hao

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关键词： mink; interferon-e; tissue distribution; antiviral activity; interferon-stimulated gene 15; MX1, 2'-5' oligoadenylate synthetase 1

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.2； 五年影响因子：3.5 ）

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年卷期： 2023 年 9 卷

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收录情况： SCI

摘要： The interferon (IFN) response is the first line of defense against viral invasion and thus plays a central role in the regulation of the immune response. IFN-epsilon (IFN-epsilon) is a newly discovered type I IFN that does not require viral induction, unlike other type I IFNs. IFN-epsilon is constitutively expressed in epithelial cells and plays an important role in mucosal immunity. In this study, we evaluated the biological activity of the mink-IFN (MiIFN)-epsilon gene in prokaryotic cells. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to evaluate IFN-epsilon expression in different mink tissues. MiIFN-epsilon was highly expressed in brain, lung, tracheal, kidney, intestinal, bladder, ovarian, and testis tissues. There was no significant difference in MiIFN-epsilon expression between female and male minks, except in the reproductive system. Expression of the small ubiquitin-like modifier (SUMO3)-MiIFN-epsilon fusion gene was induced by isopropyl(3-d-thiogalactoside, and MiIFN-epsilon was collected after SUMO-specific protease digestion. We tested the antiviral activity of MiIFN-epsilon against vesicular stomatitis virus (VSV) in epithelial cells of feline kidney 81 (F81). We used qRT-PCR to analyze the expression of several IFN-stimulated genes (ISGs), including ISG15, 2 '-5 ' oligoadenylate synthetase (2 '-5 ' OAS1), and myxovirus resistance protein 1 (Mx1). Recombinant IFN-epsilon induced high ISG expression in F81 cells. Compared with those in the cell control group, expressions of ISG15, Mx1, and 2 '-5 ' OAS1 in the VSV-GFP control, IFN-epsilon, and MiIFN-epsilon-inhibited VSV-GFP groups were significantly increased. Compared with those in the VSV-GFP control group, expressions of ISG15 and 2 '-5 ' OAS1 in the IFN-epsilon and MiIFN-epsilon-inhibited VSV-GFP groups were significantly increased, and the differences were highly significant (p < 0.0001). IFN-epsilon played an indirect antiviral role. These findings lay the foundation for detailed investigation of IFN-epsilon in the future.

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