文献类型： 外文期刊

第一作者： Zhang, Lei

作者： Zhang, Lei;Zhang, Huanmin;Zhang, Lei;Xie, Qingmei;Chang, Shuang;Ai, Yongxing;Dong, Kunzhe

作者机构： USDA ARS, US Natl Poultry Res Ctr, Athens, GA 30605 USA;Chinese Acad Agr Sci, Inst Special Wild Econ Anim & Plant Sci, Changchun 130112, Peoples R China;South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Peoples R China;Shandong Agr Univ, Coll Vet Med, Tai An 271018, Peoples R China;Jilin Univ, Coll Anim Sci, Changchun 130062, Peoples R China;Augusta Univ, Dept Pharmacol & Toxicol, Augusta, GA 30912 USA

关键词： small RNA sequencing; microRNA expression; vaccine protective efficacy; Marek's disease; bursa of Fabricius; chickens; target genes; pathways

期刊名称：VETERINARY SCIENCES （ 2022影响因子：2.4； ）

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年卷期： 2024 年 11 卷 4 期

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收录情况： SCI

摘要： Simple Summary Epigenetic factor microRNAs may modulate vaccine protective efficacy well, supported by experimental data showing that the expression of some microRNAs drastically differs between well-protected and poorly protected genetic lines of chickens. Marek's disease vaccination and virus challenge trials were conducted in two genetically divergent inbred lines of chickens, and hundreds of microRNAs were identified. However, a small number of miRNAs were observed with significant differential expression per line per vaccination treatment group. One of the vaccines was HVT, which reportedly protects the two lines of birds with a significant difference. The target genes of the differentially expressed miRNAs in response to HVT were reportedly involved with many Gene Ontology terms and pathways, which suggests there is a high complexity of the genetic/epigenetic mechanism modulating vaccine protective efficacy.Abstract Epigenetic factors, including microRNAs (miRNAs), play an important role in affecting gene expression and, therefore, are involved in various biological processes including immunity protection against tumors. Marek's disease (MD) is a highly contagious disease of chickens caused by the MD virus (MDV). MD has been primarily controlled by vaccinations. MD vaccine efficacy might, in part, be dependent on modulations of a complex set of factors including host epigenetic factors. This study was designed to identify differentially expressed miRNAs in the primary lymphoid organ, bursae of Fabricius, in response to MD vaccination followed by MDV challenge in two genetically divergent inbred lines of White Leghorns. Small RNA sequencing and bioinformatic analyses of the small RNA sequence reads identified hundreds of miRNAs among all the treatment groups. A small portion of the identified miRNAs was differentially expressed within each of the four treatment groups, which were HVT or CVI988/Rispens vaccinated line 63-resistant birds and line 72-susceptible birds. A direct comparison between the resistant line 63 and susceptible line 72 groups vaccinated with HVT followed by MDV challenge identified five differentially expressed miRNAs. Gene Ontology analysis of the target genes of those five miRNAs revealed that those target genes, in addition to various GO terms, are involved in multiple signaling pathways including MAPK, TGF-beta, ErbB, and EGFR1 signaling pathways. The general functions of those pathways reportedly play important roles in oncogenesis, anti-cancer immunity, cancer cell migration, and metastatic progression. Therefore, it is highly likely that those miRNAs may, in part, influence vaccine protection through the pathways.

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