FaNDUFB9 Attenuates Strawberry Mottle Virus Infection by Inhibiting the Activity of the Viral Gene Silencing Suppressor, Pro2Glu

文献类型： 外文期刊

第一作者： Xu, Tengfei

作者： Xu, Tengfei;Gao, Dehang;Dong, Zhenfei;Wang, Hongqing;Zhan, Binhui;Zhang, Zhixiang;Li, Shifang;Fan, Lingjiao

作者机构：

关键词： disease resistance; FaNDUFB9; RNA silencing suppressor; SMoV Pro2Glu; Strawberry mottle virus; viral infection

期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：4.9； 五年影响因子：5.6 ）

ISSN： 1464-6722

年卷期： 2025 年 26 卷 3 期

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收录情况： SCI

摘要： Strawberry mottle virus (SMoV) significantly compromises strawberry fruit quality and yield. The Pro2Glu protein of SMoV, an RNA silencing suppressor, is pivotal for viral infection; however, its interactions with host factors remain underexplored. This study identifies a critical interaction between SMoV Pro2Glu and strawberry NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 9 (FaNDUFB9). Using yeast two-hybrid assays and in vivo protein interaction analyses, we confirm that amino acid residues 31-45 of FaNDUFB9 are essential for binding Pro2Glu. Sequence analysis places FaNDUFB9 within the Complex1_LYR_superfamily, and subcellular localisation reveals its presence in both the mitochondria and nucleus. Silencing of NbNDUFB9 in Nicotiana benthamiana elevated Pro2Glu expression, whereas co-expression of FaNDUFB9 with Pro2Glu suppressed Pro2Glu levels and weakened its silencing activity. Furthermore, overexpression of FaNDUFB9 in transgenic N. benthamiana reduced transient SMoV RNA expression. These findings suggest that FaNDUFB9 inhibits expression of Pro2Glu, affecting its silencing activity, and thereby potentially further influencing viral infection.

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