First report of the whole-genome sequence analysis of Fig badnavirus 2 from China

文献类型： 外文期刊

第一作者： Aizitili, Tuxunaili

作者： Aizitili, Tuxunaili;Mijiti, Maihemuti;Aizitili, Tuxunaili;Maimaiti, Yushanjiang;Zhang, Zhixiang;Mijiti, Maihemuti

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关键词： Fig badnavirus 2; High-throughput sequence; Genomic characteristics; Phylogenetic analysis

期刊名称：VIRUS GENES （ 影响因子：1.9； 五年影响因子：1.9 ）

ISSN： 0920-8569

年卷期： 2025 年 61 卷 2 期

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收录情况： SCI

摘要： A novel plant virus was identified in fig trees exhibiting ring spot symptoms through high-throughput sequencing (HTS). The complete genome sequence was successfully determined using PCR and RT-PCR techniques. The virus features a circular DNA genome of 7233 nucleotides (nt) in length, encompassing four open reading frames (ORFs). ORF1 and ORF2 encode hypothetical proteins, while ORF3 encodes a putative polyprotein with conserved domains, including a zinc finger, aspartic protease, reverse transcriptase (RT), and RNase H. ORF4 encodes a putative protein of unknown function. Comparative nucleotide sequence analysis of the RT + RNase H region reveals 84.46% and 78.82% identity with grapevine badnavirus 1 (GBV-1, MF781082.1) and fig badnavirus 1 (FBV-1, MK348055.1), respectively. Notably, this virus's genomic organization diverges from GBV-1 but is similar to FBV-1. Phylogenetic analysis demonstrates that the three isolates of this virus form a distinct clade within the badnaviruses. Based on genomic structure and phylogenetic relationships, this novel virus represents a new member of the genus Badnavirus and is proposed to be named "Fig badnavirus 2" (FBV-2).

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