Engineering His-Tagged Senecavirus A for One-Step Purification of Viral Antigens

文献类型： 外文期刊

第一作者： Fan, Junhao

作者： Fan, Junhao;Xiao, Peiyu;Meng, Liang;An, Tongqing;Cai, Xuehui;Wang, Haiwei;Yu, Li;Kong, Dongni;Liu, Xinran

作者机构：

关键词： Senecavirus A; His-tagged virus; antigen purification

期刊名称：VACCINES （ 影响因子：4.961； 五年影响因子：5.325 ）

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年卷期： 2022 年 10 卷 2 期

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收录情况： SCI

摘要： Senecavirus A (SVA) is a picornavirus that causes vesicular disease in swine, and the inactivated vaccine is used to prevent and control SVA infection. To develop a new chromatography strategy for the purification and concentration of SVA vaccine antigens, we inserted a 6xHis-tag at the VP1 C-terminal of the SVA/HLJ/CHA/2016 in an infectious clone to rescue a His-tagged SVA. The constructed and rescued recombinant virus, named as rSVA-His, exhibited similar growth kinetics to that of its parental virus. In addition, the expression of a 6xHis-tag on the surface of SVA showed genetic stability in cell passages in vitro, which allowed one-step purification of SVA antigens by Ni2+ affinity columns. Furthermore, the immunogenicity of the inactivated rSVA-His was evaluated by inoculating rabbits and detecting neutralizing antibodies. The animals receiving two doses of the inactivated rSVA-His emulsified with oil adjuvant developed a high titer of neutralizing antibodies, indicating that SVA VP1 is tolerant to His-tag insertion without detriment to its antigenicity. In summary, the constructed 6xHis-tagged SVA may offer a feasible approach to the affinity purification and concentration of antigens in the process of SVA inactivated vaccine production.

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