Mapping of wheat stripe rust resistance gene Yr041133 by BSR-Seq analysis

文献类型: 外文期刊

第一作者: Li, Yahui

作者: Li, Yahui;Hu, Jinghuang;Shi, Xiaohan;Qiu, Dan;Wu, Peipei;Goitom, Gebremedhin Habteab;Zhang, Hongjun;Yang, Li;Liu, Hongwei;Zhou, Yang;Li, Hongjie;Wu, Qiuhong;Xie, Jingzhong;Liu, Zhiyong;Lin, Ruiming;Wang, Siqi

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关键词: Bulked segregant analysis; Genetic mapping; Puccinia striiformis f. sp. tritici; Triticum aestivum; Yellow rust

期刊名称:CROP JOURNAL ( 影响因子:4.647; 五年影响因子:5.781 )

ISSN: 2095-5421

年卷期: 2022 年 10 卷 2 期

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收录情况: SCI

摘要: Puccinia striiformis Westend. f. sp. tritici (Pst) pathotype CYR34 is widely virulent and prevalent in China. Here, we report identification of a strpie rust resistance (Yr) gene, designated Yr041133, in winter wheat line 041133. This line produced a hypersensitive reaction to CYR34 and conferred resistance to 13 other pathotypes. Resistance to CYR34 in line 041133 was controlled by a single dominant gene. Bulked segregant RNA sequencing (BSR-Seq) was performed on a pair of RNA bulks generated by pooling resistant and susceptible recombinant inbred lines. Yr041133 was mapped to a 1.7 cM genetic interval on the chromosome arm 7BL that corresponded to a 0.8 Mb physical interval (608.9-609.7 Mb) in the Chinese Spring reference genome. Based on its unique physical location Yr041133 differred from the other Yr genes on this chromosome arm. (C) 2021 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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