Screen-printed electrochemical immunosensor based on a novel nanobody for analyzing aflatoxin M-1 in milk

文献类型： 外文期刊

第一作者： Tang, Xiaoqian

作者： Tang, Xiaoqian;Huang, Xiaorong;Zhang, Qi;Li, Peiwu;Tang, Xiaoqian;Zhang, Qi;Li, Peiwu;Tang, Xiaoqian;Zhang, Qi;Li, Peiwu;Tang, Xiaoqian;Li, Peiwu;Tang, Xiaoqian;Li, Peiwu;Tang, Xiaoqian;Catanante, Gaelle;Marty, Jean-Louis;Wang, Hong

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关键词： Aflatoxin M-1; Anti-idiotypic nanobody; Chronoamperometry; Milk; Screen-printed carbon electrodes

期刊名称：FOOD CHEMISTRY （ 影响因子：9.231； 五年影响因子：8.795 ）

ISSN： 0308-8146

年卷期： 2022 年 383 卷

页码：

收录情况： SCI

摘要： This study aimed to devise a nontoxic electrochemical immunosensor to quantitatively determine aflatoxin M-1 by chronoamperometry with novel anti-idiotypic nanobody-functionalized screen-printed carbon electrodes (SPCEs). Anti-idiotype nanobodies (AIdnb) were developed to replace the high toxic chemically synthesized antigen. AIdnb was immobilized on the surface of SPCE via covalent coupling as capture reagent. The functionalized SPCEs were followed by characterization using electrochemical impedance spectroscopy, fourier-transform infrared spectroscopy, transmission electron microscopy mapping, and atomic force microscopy. After optimizing experimental parameters, the assembled immunosensor exhibited a good linearity range of 0.25-5.0 ng/mL, with the limit of detection of 0.09 ng/mL. The immunosensor showed a satisfactory selectivity to AFM1, without interference from analogs, including zearalenone, ochratoxin, and fumonisin B-1. For practical application, the developed immunosensor was validated using real spiked samples with the recovery range 82.0%-108.0% and relative standard deviation (RSD) 10.1%-13.0%, indicating that it could be used in milk samples.

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