A novel adenylate isopentenyltransferase 5 regulates shoot branching via the ATTTA motif in Camellia sinensis

文献类型： 外文期刊

第一作者： Zhang, Liping

作者： Zhang, Liping;Li, Menghan;Yan, Peng;Fu, Jianyu;Zhang, Lan;Li, Xin;Han, Wenyan

作者机构：

关键词： Adenylate isopentenyltransferase; ATTTA motif; Alternative splicing (AS) variant; Axillary bud; Cytokinins (CTKs); Tea plant

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2021 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Background Shoot branching is one of the important agronomic traits affecting yields and quality of tea plant (Camellia sinensis). Cytokinins (CTKs) play critical roles in regulating shoot branching. However, whether and how differently alternative splicing (AS) variant of CTKs-related genes can influence shoot branching of tea plant is still not fully elucidated. Results In this study, five AS variants of CTK biosynthetic gene adenylate isopentenyltransferase (CsA-IPT5) with different 3 ' untranslated region (3 UTR) and 5 UTR from tea plant were cloned and investigated for their regulatory effects. Transient expression assays showed that there were significant negative correlations between CsA-IPT5 protein expression, mRNA expression of CsA-IPT5 AS variants and the number of ATTTA motifs, respectively. Shoot branching processes induced by exogenous 6-BA or pruning were studied, where CsA-IPT5 was demonstrated to regulate protein synthesis of CsA-IPT5, as well as the biosynthesis of trans-zeatin (tZ)- and isopentenyladenine (iP)-CTKs, through transcriptionally changing ratios of its five AS variants in these processes. Furthermore, the 3 ' UTR AS variant 2 (3AS2) might act as the predominant AS transcript. Conclusions Together, our results indicate that 3AS2 of the CsA-IPT5 gene is potential in regulating shoot branching of tea plant and provides a gene resource for improving the plant-type of woody plants.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序