Preparation, identification, and preliminary application of monoclonal antibody against pyrethroid insecticide fenvalerate

文献类型： 外文期刊

第一作者： Li, Peiwu

作者： Li, Peiwu;Zhang, Qi;Li, Peiwu;Zhang, Wen;Wang, Xiupin;Li, Peiwu

作者机构：

关键词： Enzyme-linked immunosorbent assay (ELISA);Fenvalerate;Hapten;Monoclonal antibody;Pyrethroid insecticide

期刊名称：ANALYTICAL LETTERS （ 影响因子：2.329； 五年影响因子：1.738 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Monoclonal antibodies (MAbs) against pyrethroid insecticide fenvalerate were achieved, identified, and applied in environmental water. Mice were immunized with a novel synthesized hapten conjugated with bovine serum albumin (BSA). Three positive clones of MAbs were obtained after cell fusion and hybridoma selection, among them MAb-2 (5B10) showed the highest reactivity toward fenvalerate. The IC_(50) of MAb-2 was 94.5 ngmL~(-1); moreover, it showed lower cross-reactivity with other pyrethroids such as bifenthrin, tetramethrin, deltamethrin and beta-cypermethrin. Optimization of enzyme-linked immunosorbent assay (ELISA) was studied. The limit of detection (LOD) of the assay was 8.8 ngmL~(-1) and the detection range was 0.017-27.33 μgmL~(-1). For preliminary application, addition recovery experiments in water samples were performed. The mean recoveries of three kinds of samples varied from 90.6% to 108.7% and the coefficients of variation ranged from 0.5% to 5.3%. The results showed that MAb-2 could be used for the detection of fenvalerate contamination in environmental water.

分类号： O65

  • 相关文献

[1]Development of a Monoclonal Antibody-Based Enzyme Immunoassay for the Pyrethroid Insecticide Deltamethrin. Kong, Ye,Zhang, Qi,Zhang, Wen,Li, Peiwu,Zhang, Wen,Li, Peiwu,Zhang, Qi,Gee, Shirley J..

[2]Development Of A Class-specific Monoclonal Antibody-based Elisa For Aflatoxins In Peanut. Li, Peiwu,Zhang, Qi,Zhang, Wen,Zhang, Jinyang,Chen, Xiaomei,Jiang, Jun,Xie, Lihua,Zhang, Daohong.

[3]Stray dogs as indicators of Toxoplasma gondii distributed in the environment: the first report across an urban-rural gradient in China. Yan, Chao,Yue, Cai-Ling,Wang, Dong-Hui,Zhou, Dong-Hui,Yan, Chao,Fu, Lin-Lin,Yue, Cai-Ling,Tang, Ren-Xian,Liu, Yi-Sheng,Zhang, Peng,Wang, Dong-Hui,Zheng, Kui-Yang,Lv, Liang,Shi, Na,Zeng, Ping,Zhu, Xing-Quan,Zhu, Xing-Quan. 2012

[4]Seroprevalence of avian origin H3N2 canine influenza virus infection in pet dogs in Shenzhen, China. Zhao, Fu-Rong,Li, Shou-Jun,Qi, Wen-Bao,Jiao, Pei-Rong,Liao, Ming,Zhang, Gui-Hong,Zhao, Fu-Rong,Zhou, Dong-Hui,Chen, Ning,Zhang, Yan-Zhong,Tong, Guang-Zhi. 2011

[5]First report of Mycoplasma bovis infection in dairy cattle in Guangzhou, subtropical southern China. Zhao, Fu-Rong,Zhu, Xue-Liang,Xu, Min-Jun,Huang, Si-Yang,Zhou, Dong-Hui,Xia, Hui-Yan,Song, Hui-Qun,Xia, Hui-Yan,Song, Hui-Qun,Zou, Feng-Cai. 2012

[6]Bt RICE PRODUCING Cry1C PROTEIN DOES NOT HAVE DIRECT DETRIMENTAL EFFECTS ON THE GREEN LACEWING CHRYSOPERLA SINICA ( TJEDER). Chen, Xiuping,Hu, Long,Romeis, Joerg,Peng, Yufa,Romeis, Joerg. 2014

[7]Anti-oxytetracycline monoclonal antibody based detection of oxytetracycline residue in honey. Xu, Shufa,Hou, Liwei,Wubie, Abebe J.,Cao, Tan,Hu, Yingying,Li, Wei,Guo, Zhanbao,Zhao, Jing,Wu, Jie,Hou, Liwei,Zhao, Jing. 2013

[8]Resistance of Helicoverpa assulta (Guenee) (Lepidoptera: Noctuidae) to fenvalerate, phoxim and methomyl in China. Wang, Kai Yun,Xia, Xiao Ming,Wang, Hong Yan. 2009

[9]Paternal Fenvalerate Exposure Influences Reproductive Functions in the Offspring. Xia, Dong,Xu, Kesi,Jiang, Hui,Li, Rongjie,Hang, Yiqiong,Lu, Yang,Parvizi, Nahid,Zhou, Yuchuan. 2013

[10]Selective sample cleanup by immunoaffinity chromatography for determination of fenvalerate in vegetables. Wang, Yanru,Zhang, Qi,Li, Peiwu,Zhang, Wen,Li, Ying,Ding, Xiaoxia,Wang, Yanru,Li, Peiwu,Ding, Xiaoxia,Zhang, Qi,Wang, Yanru,Li, Peiwu,Zhang, Wen,Li, Ying,Ding, Xiaoxia.

[11]Cloning and expression of cytochrome P450 CYP6B7 in fenvalerate-resistant and susceptible Helicoverpa armigera (Hubner) from China. Tang, T.,Cheng, Y.,Shui, R.,Zhang, W.,Qiu, L.,Zhang, H.,Tang, T.. 2010

[12]CHARACTERIZATION OF SOLUBLE AND MEMBRANE-BOUND ALKALINE PHOSPHATASE IN Nilaparvata lugens AND THEIR POTENTIAL RELATION TO DEVELOPMENT AND INSECTICIDE RESISTANCE. Yang, Baojun,Wang, Zengxia,Liu, Zewen. 2011

[13]Mechanism of resistance alleviation in Helicoverpa armigera (Lepidoptera : Noctuidae) to pyrethroid caused by Bacillus thuringiensis pretreatment. Liang, GM,Guo, YY. 1998

[14]Knockdown of several components of cytochrome P450 enzyme systems by RNA interference enhances the susceptibility of Helicoverpa armigera to fenvalerate. Zhao, Chunqing,Feng, Xiaoyun,Qiu, Lihong,Tang, Tao,Liu, Xueyuan. 2012

[15]Sub-Lethal Effects of Fenvalerate on the Development, Fecundity, and Juvenile Hormone Esterase Activity of Diamondback Moth, Plutella xylostella (L.). Gu Xiao-jun,Wei Hui,Wei Hui,Wang Juan,Li Hong-shan,Dai Hua-guo,Li Hong-shan. 2010

[16]Resistance alleviation in the larval cotton bollworm to fenvalerate after pretreatment with bacillus thuringiensis. 梁革梅,郭予元. 1999

[17]Antibody Developments and Immunoassays for Organophosphorus Chemicals: A Review. Hua, Xiude,Liu, Fengquan,Wang, Minghua,Hua, Xiude,Liu, Fengquan,Wang, Minghua,Eremin, Sergei A.,Eremin, Sergei A.,Liu, Fengquan. 2017

[18]Developments on Immunoassays for Pyrethroid Chemicals. Jin, Maojun,Chen, Ge,Du, Pengfei,Zhang, Chan,Cui, Xueyan,She, Yongxin,Zheng, Lufei,Wang, Shanshan,Shao, Hua,Jin, Fen,Wang, Jing,Gee, Shirley J.,Hammock, Bruce D.,Gee, Shirley J.,Hammock, Bruce D.. 2017

[19]Prepared polyclonal antibody of the semicarbazide. Huang, Xuan-yun,Huang, Dong-mei,Cai, You-qiong. 2015

[20]Development of an enzyme-linked immunosorbent assay for podophyllotoxin. Xu, DM,Yu, XY,Liu, YQ,Feng, JT,Pan, LG,Liu, XJ,He, J,Zhang, X. 2005

作者其他论文 更多>>

微信小程序