Kitchen Waste Oil Convert to Biodiesel via W/O Interface Biocatalysis with Thermomyces Lanuginosus Lipase-PNIPAAm Conjugates

文献类型： 外文期刊

第一作者： Shi, Can-Yang

作者： Shi, Can-Yang;Hu, Yan;Chai, Ya-Li;Wu, Cheng-Kun;Wu, Ji-Long;You, Shuai;Wang, Jun;Wang, Jun

作者机构：

关键词： Biodiesel; Grafting-from; Kitchen waste oil; TL-PNIPAAm conjugate; Oil/water interface catalysis

期刊名称：WASTE AND BIOMASS VALORIZATION （ 影响因子：3.449； 五年影响因子：3.575 ）

ISSN： 1877-2641

年卷期： 2022 年 13 卷 9 期

页码：

收录情况： SCI

摘要： Due to the scarcity of resources, alternatives to non-renewable resources have become more important. Chemical catalysts react violently and the conditions are harsh. Using biocatalysts at the water/oil (W/O) interface remains technically challenging and is rewarding. In this study, the Thermomyces lanuginosus lipase-poly coupling biocatalyst was prepared by the atom transfer radical polymerization "grafting-from" method. The TL-PNIPAAm conjugate was successfully prepared and circular dichroism showed a negligible change between the free enzyme and conjugated enzyme by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transmission electron microscopy. Furthermore, the temperature and pH stability of the conjugate was better than that of free TL. The remaining activity of the TL-PNIPAAm conjugate for 10 h was approximately 20% higher than that of free TL after heat treatment (55 degrees C) and higher pH stability was obtained in the pH 4-10. The remaining activity of the TL-PNIPAAm conjugates was above 60% after recycling 7 times. In addition, the conversion of biodiesel from kitchen waste oil was 92.6% at 50 degrees C for 25 h with a conversion efficiency of 0.04 g/mL/h. Thus, a high stability and reusability biocatalyst and stabilizer at the interface of water-in-oil Pickering emulsions was successfully prepared.

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