Fine Mapping of a Foot-and-mouth Disease Virus Epitope Recognized by Serotype-Independent Monoclonal Antibody 4B2

文献类型： 外文期刊

第一作者： Yu, Yongzhong

作者： Yu, Yongzhong;Wang, Haiwei;Zhao, Lei;Zhang, Chunyuan;Jiang, Zhigang;Yu, Li

作者机构：

关键词： Epitope Mapping;Foot-and-mouth Disease Virus;Monoclonal Antibody;Phage Display;VP2

期刊名称：JOURNAL OF MICROBIOLOGY （ 影响因子：3.422； 五年影响因子：3.28 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： VP2 is a structural protein of the foot-and-mouth disease virus (FMDV). In this study, a FMDV serotype-in-dependent monoclonal antibody (MAb), 4B2, was generated. By screening a phage-displayed random 12-peptide library, we found positive phages displaying the consensus motif ETTXLE (X is any amino acid (aa)), which is highly homologous to ~6ETTLLE~(11) at the N-terminus of the VP2 protein. Subsequently, a series of GST-fusion proteins expressing a truncated N-terminus of VP2 were examined by western blot analysis using the MAb 4B2. The results indicated that the motif _6ETTLLE~(11) of VP2 may be the minimal requirement of the epitope recognized by 4B2. Moreover, a 12-aa peptide ~2KKTEETTLLEDR~(13) was shown to be the minimal unit of the epitope with maximal binding activity to 4B2. Alanine-scanning analysis demonstrated thatThr~7, Thr~8, and Leu~(10) are the functional residues of the 4B2 epitope Glu6 and Leu9 are required residues, and Glu11 plays a crucial role in the binding of MAb 4B2. The fine mapping of the epitope indicated that MAb 4B2 has the potential to be used in FMDV diagnosis.

分类号： Q93

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