Callus induction and plant regeneration in Alternanthera philoxeroides

文献类型: 外文期刊

第一作者: Gao, Jianming

作者: Gao, Jianming;Li, Jinzhi;Luo, Caibo;Yin, Liang;Li, Shengchun;Yang, Guangxiao;He, Guangyuan;Gao, Jianming;Li, Jinzhi

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关键词: Alternanthera philoxeroides (Mart.) Griseb;Callus induction;Orthogonal design;Plantlet regeneration

期刊名称:MOLECULAR BIOLOGY REPORTS ( 影响因子:2.316; 五年影响因子:2.357 )

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收录情况: SCI

摘要: To establish a successful in vitro plant regeneration system in Alternanthera philoxeroides (Mart.) Griseb, an orthogonal design was used to investigate the effects of three factors (plant growth regulators, explant types and dark treatment in initial-stage), each having three levels. The effects of these factors and levels on callus induction and shoot regeneration were quantitatively evaluated by analysis of variance. The experimental results showed that the callus induction was significantly affected by 2, 4-dichlorophenoxyacetic acid (2, 4-D), and shoot differentiation from subcultured pieces of callus was enhanced mostly by dark treatment in initial-stage. The optimal conditions for callus induction are obtained from the stem explants cultured on semi-solid Murashige and Skoog (MS) medium plus 2.2 mu M BA and 2.2 mu M 2, 4-D, with 20days dark treatment in initial-stage. The highest frequency of shoot regeneration is obtained from the calli cultured on semi-solid MS medium plus 8.8 mu M BA, without dark treatment in initial-stage.

分类号: Q7

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