Percoll gradient separation of cryopreserved common carp spermatozoa to obtain a fraction with higher motility, velocity and membrane integrity

文献类型： 外文期刊

第一作者： Li, Ping

作者： Li, Ping;Dzyuba, Boris;Hulak, Martin;Rodina, Marek;Boryshpolets, Sergey;Li, Zhi-Hua;Linhart, Otomar;Li, Ping;Li, Zhi-Hua;Dzyuba, Boris

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关键词： fertilizing ability;membrane integrity;cryobiological research;higher motility;higher velocity

期刊名称：THERIOGENOLOGY （ 影响因子：2.74； 五年影响因子：2.93 ）

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收录情况： SCI

摘要： We attempted to select a fraction of common carp, Cyprinus curpio spermatozoa that best survived a conventional freeze/thaw procedure, by centrifugation of frozen/thawed sperm through a Percoll gradient (45% and 90%). The proportion of motile spermatozoa (65.81 +/- 5.19%), their velocity (77.58 +/- 31.07 mu m/sec), and membrane integrity (83.66 +/- 4.38% intact) were significantly higher in separated sperm than in whole samples (motility 23.36 +/- 2.98%, velocity 55.55 +/- 19.03 mu m/sec, and membrane integrity 57.92 +/- 4.65%). Our results demonstrated that Percoll gradient centrifugation shows promise as a technique for selecting high quality cryopreserved fish spermatozoa, which could be useful for cryobiological research. Further studies are needed to evaluate the potentially higher fertilizing ability of the separated spermatozoa

分类号： Q95

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