Transcriptome analysis and functional study of phospholipase A2 in Galleria mellonella larvae lipid metabolism in response to envenomation by an ectoparasitoid, Iseropus kuwanae

文献类型： 外文期刊

第一作者： Zhu, Hanqi

作者： Zhu, Hanqi;Liang, Xinhao;Ding, Jianhao;Wang, Jinzheng;Li, Ping;Zhou, Weihong;Wang, Jun;Wu, Fu-an;Sheng, Sheng;Li, Ping;Zhou, Weihong;Wang, Jun;Wu, Fu-an;Sheng, Sheng

作者机构：

关键词： ectoparasitoids; Galleria mellonella; Iseropus kuwanae; lipid metabolism; the greater wax moth; transcriptome; wasp development

期刊名称：INSECT SCIENCE （ 影响因子：3.0； 五年影响因子：3.5 ）

ISSN： 1672-9609

年卷期： 2025 年 32 卷 3 期

页码：

收录情况： SCI

摘要： There is abundant evidence that parasitoids manipulate their hosts by envenomation to support the development and survival of their progeny before oviposition. However, the specific mechanism underlying host nutritional manipulation remains largely unclear. To gain a more comprehensive insight into the effects induced by the gregarious ectoparasitoid Iseropus kuwanae (Hymenoptera: Ichneumonidae) on the greater wax moth Galleria mellonella (Lepidoptera: Pyralidae) larvae, we sequenced the transcriptome of both non-envenomed and envenomed G. mellonella larvae, specifically targeting genes related to lipid metabolism. The present study revealed that 202 differentially expressed genes (DEGs) were identified and 9 DEGs were involved in lipid metabolism. The expression levels of these 9 DEGs relied on envenomation and the duration post-envenomation. Further, envenomation by I. kuwanae induced an increase in triglyceride (TG) level in the hemolymph of G. mellonella larvae. Furthermore, silencing GmPLA(2) in G. mellonella larvae 24 h post-envenomation significantly decreased the content of 4 unsaturated fatty acids and TG levels in the hemolymph. The content of linoleic acid and alpha-linoleic acid were significantly decreased and the content of oleic acid was significantly increased by exogenous supplement of arachidonic acid. Meanwhile, the reduction in host lipid levels impairs the growth and development of wasp offspring. The present study provides valuable knowledge about the molecular mechanism of the nutritional interaction between parasitoids and their hosts and sheds light on the coevolution between parasitoids and host insects.

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