Effect of combined cold pretreatment and heat shock on microspore cultures in broccoli

文献类型： 外文期刊

第一作者： Yuan, S. X.

作者： Yuan, S. X.;Liu, Y. M.;Fang, Z. Y.;Yang, L. M.;Zhuang, M.;Zhang, Y. Y.;Sun, P. T.

作者机构：

关键词： genotype;embryogenesis;cold pretreatment;diploid frequency

期刊名称：PLANT BREEDING （ 影响因子：1.832； 五年影响因子：1.956 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： P>Applied stress is often used to induce embryogenesis in microspore culture. Cold pretreatment has been used in cereal crops, Brassica napus and Brassica rapa microspore cultures, but seldom attempted in Brassica oleracea microspore culture. In this study, the effect of cold pretreatment (4 degrees C) on embryogenesis in microspore culture and ploidy of regenerants resulting from those cultures was investigated in three broccoli cultivars 'Green Elite', 'Green Star' and 'TI-111'. Cold pretreatment for 1, 2 or 3 days was effective when applied alone using these three cultivars, but the frequency of embryos derived from microspores was low. The combination of cold pretreatment (4 degrees C) for 1 or 2 days and heat shock (32.5 degrees C) for 1 day significantly enhanced microspore embryogenesis efficiency, especially with 'TI-111' for which it was increased by 63-72-fold. In addition, the combined treatment resulted in higher diploid frequency of the regenerated population compared to traditional microspore culture protocol which typical only uses heat shock at 32.5 degrees C for 1 day in broccoli.

分类号： S3

  • 相关文献

[1]Induction and development of microspore-derived embryos in broccoli x white-headed cabbage hybrids microspore culture. Zeng, Aisong,Song, Lixiao,Liu, Huanhuan,Hou, Xilin,Li, Ying,Zeng, Aisong,Yan, Jiyong,Song, Lixiao,Gao, Bing,Zhang, Yunxia,Li, Jianqi.

[2]Efficient doubled haploid production in microspore culture of loose-curd cauliflower (Brassica oleracea var. botrytis). Gu, Honghui,Zhao, Zhengqing,Sheng, Xiaoguang,Yu, Huifang,Wang, Jiansheng. 2014

[3]Cold pretreatment enhances microspore embryogenesis in oilseed rape (Brassica napus L.). Gu, HH,Hagberg, P,Zhou, WJ.

[4]Small RNA profiling and degradome analysis reveal regulation of microRNA in peanut embryogenesis and early pod development. Gao, Chao,Wang, Pengfei,Zhao, Shuzhen,Zhao, Chuanzhi,Xia, Han,Hou, Lei,Zhang, Ye,Li, Changsheng,Wang, Xingjun,Wang, Xingjun,Ju, Zheng. 2017

[5]Genome-wide analysis of WOX genes in upland cotton and their expression pattern under different stresses. Zhaoen Yang,Qian Gong,Wenqiang Qin,Qin, Wenqiang,Yang, Zhaoen,Zuoren Yang,Yuan Cheng,Lili Lu,Xiaoyang Ge,Chaojun Zhang,Zhixia Wu,Fuguang Li. 2017

[6]Plant development from microspore-derived embryos in oilseed rape as affected by chilling, desiccation and cotyledon excision. Zhang, GQ,Zhang, DQ,Tang, GX,He, Y,Zhou, WJ. 2006

[7]High frequency spontaneous production of doubled haploid plants in microspore cultures of Brassica rapa ssp chinensis. Gu, HH,Zhou, WJ,Hagberg, P. 2003

[8]Effects of pH, MES, arabinogalactan-proteins on microspore cultures in white cabbage. Yuan, Su-xia,Su, Yan-bin,Liu, Yu-mei,Fang, Zhi-yuan,Yang, Li-mei,Zhuang, Mu,Zhang, Yang-yong,Sun, Pei-tian.

[9]Improvement of Agrobacterium-mediated transformation efficiency and transgenic plant regeneration of Vitis vinifera L. by optimizing selection regimes and utilizing cryopreserved cell suspensions. Li, P,Hanania, U,Sahar, N,Mawassi, M,Gafny, R,Sela, I,Tanne, E,Perl, A.

[10]Cloning, molecular characterization and expression analysis of heat shock cognate 70 (Hsc70) cDNA from turbot (Scophthalmus maximus). Wang, T. T.,Wang, N.,Liao, X. L.,Meng, L.,Liu, Y.,Chen, S. L.,Wang, T. T..

[11]Cloning, genomic structure and expression analysis of ubc9 in the course of development in the half-smooth tongue sole (Cynoglossus semilaevis). Hu, Qiaomu,Chen, Songlin,Hu, Qiaomu.

[12]Hunchback IS REQUIRED FOR ABDOMINAL IDENTITY SUPPRESSION AND GERMBAND GROWTH IN THE PARTHENOGENETIC EMBRYOGENESIS OF THE PEA APHID, Acyrthosiphon pisum. Liu, Changyan,Zeng, Fanrong.

[13]Improvement of isolated microspore culture of barley (Hordeum vulgare L.): the effect of floret co-culture. Lu, Ruiju,Wang, Yifei,Sun, Yuefang,Shan, Lili,Huang, Jianhua,Lu, Ruiju,Wang, Yifei,Sun, Yuefang,Shan, Lili,Huang, Jianhua,Lu, Ruiju,Chen, Peidu.

[14]Studies on Agrobacterium-mediated genetic transformation of embryogenic suspension cultures of sweet potato. Xing, Yu-Jun,Ji, Qin,Luo, Yu-Ming,Xing, Yu-Jun,Yang, Qing,Li, Qiang,Wang, Xin. 2008

[15]Anther culture and plant regeneration of cotton (Gossypium klotzschianum Anderss). Feng, R,Li, XH,Li, FL. 1996

[16]Morphological anatomy, sporogenesis and gametogenesis in flowering process of jasmine (Jasminum sambac Aiton). Deng, Yanming,Jia, Xinping,Liang, Lijian,Sun, Xiaobo,Gu, Chunsun.

[17]Embryo induction and plant regeneration of Callerya speciosa (Fabaceae) through anther culture. Huang, Bilan,Xu, Li,Li, Kelie,Fu, Yunlu,Li, Zhiying.

[18]Molecular characterization of the growth hormone in Chinese sturgeon and its expression during embryogenesis and early larval stages. Cao, H.,Gui, J. F.,Cao, H.,Zhou, R. J.,Wei, Q. W.,Li, C. J..

[19]Molecular cloning, characterization and expression analysis of a clip-domain serine protease from pearl oyster Pinctada fucata. Xu, Xinping,Xu, Xinping,Zhang, Dianchang,Jiang, Shigui,Ma, Jianjun,Jiang, Jingjing,Pan, Dequan,Zhang, Dianchang,Jiang, Jingjing.

[20]Screening of genes induced by salt stress from Alfalfa. Jin, Houcong,Yang, Qingchuan,Chao, Yuehui,Kang, Junmei,Li, Yan,Sun, Yan,Jin, Houcong,Jin, Hong,Margaret, Gruber.

作者其他论文 更多>>

微信小程序