Simultaneous estimation of vinpocetine and its metabolite, apovincaminic acid, in beagle plasma by LC-MS-MS

文献类型： 外文期刊

第一作者： Yang, Yuan

作者： Yang, Yuan;Zhang, Kai-Chun;Yang, Yuan;Lou, Kun;Gong, Wei;Mei, Xing-Guo;Lou, Kun

作者机构：

关键词： Apovincaminic acid;Column liquid chromatography-mass spectrometry;Multiple reaction monitoring;Vinpocetine

期刊名称：CHROMATOGRAPHIA （ 影响因子：2.044； 五年影响因子：1.725 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A method was developed to determine vinpocetine and its metabolite, apovincaminic acid, in beagle plasma by LC-MS-MS. After protein precipitation with methanol, the supernatant of the sample was concentrated and injected into an Agilent Zorbax XDB-C_(18) column. The sample was separated by a mobile phase consisting of acetonitrile and 0.2% formic acid solution, and the reading was determined on an Agilent 6410 Triple Quad Tandem mass spectrometer in multiple reaction monitoring mode with the following transitions: m/z 351.5 → 280.2/266.3 for vinpocetine, 323.2 → 236.1/280.2 for apovincaminic acid, and 411.2 → 191.1 for the internal standard. The intra- and inter-day variances were less than 15% (RSD%), and average recoveries were higher than 80%. The linearity ranges (LR) between 0.1 and 20.0 ng mL~(-1) for vinpocetine (r ~2 = 0.9980) and between 1.0 and 200.0 ng mL ~(-1) for apovincaminic acid (r ~2 = 0.9995) were established. In summary, this method is sensitive, specific, and appropriate for in vivo study of various dosage forms of vinpocetine.

分类号： O65

  • 相关文献

[1]Metabolic Profile of Nitazoxanide in Goat Feces. Zhao, Zhanzhong,Zhang, Lifang,Xue, Feiqun,Zhang, Tao.

[2]In-depth proteome analysis of the rubber particle of Hevea brasiliensis (para rubber tree). Dai, Longjun,Kang, Guijuan,Li, Yu,Nie, Zhiyi,Duan, Cuifang,Zeng, Rizhong,Dai, Longjun,Kang, Guijuan,Li, Yu,Nie, Zhiyi,Duan, Cuifang,Zeng, Rizhong.

[3]Determination of Pentachloronitrobenzene and Its Metabolites in Ginseng by Gas Chromatography-Ion Trap Tandem Mass Spectrometry. Li Chong-Jiu,Dong Feng-Shou,Liu Xin-Gang,Zheng Yong-Quan,Li Jing. 2008

作者其他论文 更多>>

微信小程序