Enhancement of artemisinin content in tetraploid Artemisia annua plants by modulating the expression of genes in artemisinin biosynthetic pathway

文献类型： 外文期刊

第一作者： Lin, Xiuyan

作者： Lin, Xiuyan;Lu, Xu;Zhang, Fangyuan;Shen, Qian;Wu, Shaoyan;Chen, Yunfei;Wang, Tao;Tang, Kexuan;Zhou, Yin;Zhang, Jianjun

作者机构：

关键词： Artemisia annua L.;Colchicine;Flow cytometry;Gene expression;Stomata;Tetraploid

期刊名称：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY （ 影响因子：2.431； 五年影响因子：2.124 ）

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收录情况： SCI

摘要： Tetraploid Artemisia annua plants were successfully inducted by using colchicine, and their ploidy was confirmed by flow cytometry. Higher stomatal length but lower frequency in tetraploids were revealed and could be considered as indicators of polyploidy. The average level of artemisinin in tetraploids was increased from 39% to 56% than that of the diploids during vegetation period, as detected by high-performance liquid chromatography-evaporative light scattering detector. Gene expressions of 10 key enzymes related to artemisinin biosynthetic pathway in different ploidy level were analyzed by semiquantitative polymerase chain reaction and significant upregulation of FPS, HMGR, and artemisinin metabolite-specific Aldh1 genes were revealed in tetraploids. Slight increased expression of ADS was also detected. Our results suggest that higher artemisinin content in tetraploid A. annua may result from the upregulated expression of some key enzyme genes related to artemisinin biosynthetic pathway.

分类号： Q81

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