Simultaneous determination of 13 phytohormones in oilseed rape tissues by liquid chromatography-electrospray tandem mass spectrometry and the evaluation of the matrix effect

文献类型： 外文期刊

第一作者： Fan, Sufang

作者： Fan, Sufang;Wang, Xiupin;Li, Peiwu;Zhang, Qi;Zhang, Wen;Fan, Sufang;Wang, Xiupin;Li, Peiwu;Zhang, Qi;Zhang, Wen;Fan, Sufang;Wang, Xiupin;Li, Peiwu

作者机构：

关键词： Liquid chromatography-tandem mass spectrometry;Matrix effect;Phytohormones;Rape tissues

期刊名称：JOURNAL OF SEPARATION SCIENCE （ 影响因子：3.645； 五年影响因子：2.943 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In the experiment, a high-performance liquid chromatography and electrospray ionization-tandem mass spectrometry with selected reaction monitoring was used to simultaneously determine various classes of phytohormones, including indole-3-acetic acid, α-naphthaleneacetic acid, 2-chlorobenzoic acid, 4-chlorobenzoic acid, indole-3-butyric acid, gibberellic acid, 2,4-dichlorophenoxyacetic acid, 2-naphthoxyacetic acid, abscisic acid, 2,3,5-triiodobenzoic acid, uniconazole, paclobutrazol and 2,4-epibassinolide in rape tissues. The analyses were separated by an HPLC equipped with a reversed-phase column using a binary solvent system composed of methanol and water, both containing 0.1% of formic acid. The matrix effect was also considered and determined. The technology was applied to analyze rape tissues, including roots, stems, leaves, flowers, immature pods and rape seeds. The rape tissues were subjected to ultrasound-assisted extraction and purified by dispersive solid-phase extraction, and then transferred into the liquid chromatography system. The detection limit for each plant hormone was defined by the ratio of signal/background noise (S/N) of 3. The results showed perfect linearity (R~2 values of 0.9987-1.0000) and reproducibility of elution times (relative standard deviations, RSDs,<1%) and peak areas (RSDs,<7%) for all target compounds.

分类号： O6

  • 相关文献

[1]The Determination of PAHs in Fish by GC-QqQ-MS/MS. Li, Hui-Dong,Wang, Wen-Bo,Chen, Zi-Lei,Ding Ruiyan,Fang Liping,Wang Fengen,Lu, Xiao,Huo, Lu-Ge,Yang, Guo-Sheng,Li, Hui-Dong,Wang, Wen-Bo,Chen, Zi-Lei,Ding Ruiyan,Fang Liping,Wang Fengen,Lu, Xiao,Li, Hui-Dong,Wang, Wen-Bo,Chen, Zi-Lei,Ding Ruiyan,Fang Liping,Wang Fengen,Lu, Xiao,Aboul-Enein, Hassan Y.. 2013

[2]Validated hydrophilic interaction LC-MS/MS method for determination of N-methyl-2-pyrrolidinone residue. Applied to a depletion study of N-methyl-2-pyrrolidinone in swine liver following intramuscular administration of drug N-methyl-2-pyrrolidinone formulation. Liu, Yanhong,Ji, Yanhong,Chen, Jingtao,Chen, Ximing,Wang, Guangxiang,Shang, Youjun,Liu, Xiangtao,Pan, Jack. 2010

[3]Rapid and accurate determination of dichlorvos in water by liquid chromatography-electrospray ionization-tandem mass spectrometry. Yang, Lan,Liu, Yan Hong,An, Li Zhe,Li, You-bin.

[4]Phage-Displayed Peptide That Mimics Aflatoxins and Its Application in Immunoassay. Wang, Yanru,Zhang, Qi,Wang, Yanru,Li, Peiwu,Wang, Yanru,Li, Peiwu,Zhang, Qi,Wang, Yanru,Li, Peiwu,Wang, Hong,Kim, Hee Jou,Gee, Shirley J.,Hammock, Bruce D.,Wang, Hong,Kim, Hee Jou,Gee, Shirley J.,Hammock, Bruce D..

[5]Validated hydrophilic interaction LC-MS/MS method for simultaneous quantification of dacarbazine and 5-amino-4-imidazole-carboxamide in human plasma. Yang, Yuhui,Zhang, Weihua,Liu, Yanhong.

[6]Validated hydrophilic interaction LC-MS/MS method for determination of 2-pyrrolidinone residue: Applied to a depletion study of 2-pyrrolidinone in swine liver. Liu, Yanhong,Chen, Ximing,Ji, Yanhong,Chen, Jiangtao,Wang, Guangxiang,Shang, Youjun,Liu, Xiangtao,Pan, Jack.

[7]Determination of Multi-Class Mycotoxins in Tartary Buckwheat by Ultra-Fast Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry. Ren, Guixing,Hu, Yichen,Zou, Liang,Zhao, Gang,Ren, Guixing,Zhang, Jinming. 2018

[8]Simultaneous determination of aflatoxin M-1, ochratoxin A, zearalenone and alpha-zearalenol in milk by UHPLC-MS/MS. Huang, L. C.,Zheng, N.,Wen, F.,Cheng, J. B.,Han, R. W.,Xu, X. M.,Li, S. L.,Wang, J. Q.,Huang, L. C.,Cheng, J. B.,Zheng, B. Q.,Huang, L. C.,Zheng, N.,Wen, F.,Han, R. W.,Xu, X. M.,Li, S. L.,Wang, J. Q.,Wang, J. Q.. 2014

[9]Detection of ractopamine residues in pork by surface plasmon resonance-based biosensor inhibition immunoassay. Zheng, Hong,Yang, Guo-Sheng,Lu, Xiao,Li, Xiu-Qing,Yuan, Xue-Xia,Deng, Li-Gang,Zhang, Hong,Wang, Wen-Zheng,Lu, Xiao,Li, Xiu-Qing,Yuan, Xue-Xia,Deng, Li-Gang,Zhang, Hong,Wang, Wen-Zheng,Yang, Guo-Sheng,Li, Hui,Meng, Meng,Xi, Ri-Mo,Aboul-Enein, Hassan Y..

[10]A sensitive chemiluminescence enzyme immunoassay based on molecularly imprinted polymers solid-phase extraction of parathion. Chen, Ge,Jin, Maojun,Du, Pengfei,Zhang, Chan,Cui, Xueyan,Zhang, Yudan,She, Yongxin,Shao, Hua,Jin, Fen,Wang, Shanshan,Zheng, Lufei,Wang, Jing.

[11]Relationships between endogenous hormonal content and direct somatic embryogenesis in Watermelon (Citrullus lanatus) cotyledons. Zhang, Hui Jun,Wang, Qing,Wang, Qing. 2015

[12]Abiotic Stresses and Phytohormones Regulate Expression of FAD2 Gene in Arabidopsis thaliana. Yuan Si-wei,Wu Xue-long,Liu Zhi-hong,Huang Rui-zhi,Yuan Si-wei,Luo Hong-bing. 2012

[13]Regulatory mechanisms of oxidative species and phytohormones in marine microalgae Isochrysis zhangjiangensis under nitrogen deficiency. Wu, Shuang,Meng, Yingying,Cao, Xupeng,Xue, Song,Wu, Shuang. 2016

[14]Carbon nanomaterials alter plant physiology and soil bacterial community composition in a rice-soil-bacterial ecosystem. Hao, Yi,Zhang, Zetian,Rui, Yukui,Liu, Liming,Ma, Chuanxin,Rui, Yukui,Xing, Baoshan,Ma, Chuanxin,Song, Youhong,Cao, Weidong,Zhou, Guopeng,Guo, Jing. 2018

[15]Further insight into the role of KAN1, a member of KANADI transcription factor family in rice. Adedze, Yawo Mawunyo Nevame,Feng, Baobing,Shi, Lei,Sheng, Zhonghua,Tang, Shaoqing,Wei, Xiangjin,Hu, Peisong. 2018

[16]Agrobacterium-mediated genetic transformation of peanut and the efficient recovery of transgenic plants. Chen, Mingna,Yang, Qingli,Wang, Tong,Chen, Na,Pan, Lijuan,Chi, Xiaoyuan,Yang, Zhen,Wang, Mian,Yu, Shanlin,Chi, Xiaoyuan. 2015

[17]Profiling of phytohormones and their major metabolites in rice using binary solid-phase extraction and liquid chromatography-triple quadrupole mass spectrometry. Cao, Zhao-Yun,Mou, Ren-Xiang,Zhang, Lin-Ping,Lin, Xiao-Yan,Zhu, Zhi-Wei,Chen, Ming-Xue,Sun, Li-Hua.

[18]Combined transcriptome and metabolome analyses to understand the dynamic responses of rice plants to attack by the rice stem borer Chilo suppressalis (Lepidoptera: Crambidae). Wang, Xingyun,Romeis, Jorg,Peng, Yufa,Li, Yunhe,Tzin, Vered,Romeis, Jorg. 2016

[19]The Influence of Endogenous Hormones on Somatic Embryos Formation from Anthers of Hevea Brasiliensis Muell. Arg. Huijun, Z.,Xianfeng, Y.,Huasun, H.,Tiandai, H.,Quannan, Z.. 2016

[20]OsARF16, a transcription factor regulating auxin redistribution, is required for iron deficiency response in rice (Oryza sativa L.). Shen, Chenjia,Sun, Tao,Yang, Yanjun,Wang, Huizhong,Yue, Runqing,Zhang, Lei.

作者其他论文 更多>>

微信小程序