Immunological regulation by Toll-1 and Spatzle-4 in larval density-dependent prophylaxis of the oriental armyworm, Mythimna separata

文献类型： 外文期刊

第一作者： Jiang, Suwan

作者： Jiang, Suwan;Bao, Jianqiang;Chen, Yuxuan;Liu, Zhonglin;Liu, Rui;Kong, Hailong;Cheng, Yunxia;Zhang, Lei;Jiang, Xingfu

作者机构： Yangzhou Univ, Coll Plant Protect, Wenhui East Rd 48, Yangzhou 225009, Peoples R China;Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, 2 West Yuanmingyuan Rd, Beijing 100193, Peoples R China

关键词： Mythimna separata; Prophylactic immunity; Toll-Spatzle pathway

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 2022影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2024 年 264 卷

页码：

收录情况： SCI

摘要： High population density has been shown to alter insect prophylactic immunity. Toll-Spatzle pathway performs a key function in insect innate immune response. To determine the role of Toll and Spatzle, two main components of Toll-Spatzle pathway, in the density-dependent prophylaxis of Mythimna separata. We identified full-length cDNA encoding the Toll-1 and Spatzle-4 genes in M. separata (designed MsToll-1 and Ms Spatzle-4). Both MsToll-1 and MsSpatzle-4 were expressed throughout all developmental stages. MsToll-1 expression was highly in fat body and brain and MsSpatzle-4 was highly expressed in brain and Malpighian tubule. With increased larval density, MsToll-1 expression was markedly up-regulated. MsSpatzle-4 expression was found to be raised in larvae that were fed in high density (5 and 10 larvae per jar). Co-immunoprecipitation assays demonstrated that MsToll1 interacted with MsSpatzle-4. Immune-related genes transcriptions were considerably reduced in high-density larvae MsToll-1 (or MsSpatzle-4) was silenced by dsRNA injection. Meanwhile, a discernible reduction in the survival rate of the larvae exposed to Bacillus thuringiensis infection with silence of MsToll-1 (or MsSpatzle-4) was observed. This study implies that prophylactic immunity was influenced by crowded larvae via modulating the Toll-Spatzle pathway in M. separata and allow for a new understanding of into density-dependent prophylaxis in insects.

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