Characterization of a Glucose-, Xylose-, Sucrose-, and d-Galactose-Stimulated beta-Glucosidase from the Alkalophilic Bacterium Bacillus halodurans C-125
文献类型: 外文期刊
第一作者: Xu, Hu
作者: Xu, Hu;Chen, Jian-Min;Xu, Hu;Xiong, Ai-Sheng;Zhao, Wei;Tian, Yong-Sheng;Peng, Ri-He;Yao, Quan-Hong
作者机构:
关键词: magnesium ion: 22537-22-0;open reading frame: ORF;cadmium ion: 22537-48-0;zinc ion: 23713-49-7;ferric ion: 20074-52-6;o-nitrophenyl-beta-D-glucopyranoside;glucose: 58367-01-4;sugar;sucrose: 57-50-1;sugar;xylose: 25990-60-7;sugar;453-amino acid protein: glycoside hydrolase family 1;D-galactose: 59-23-4;sugar;enzyme activity;alkaline condition
期刊名称:CURRENT MICROBIOLOGY ( 影响因子:2.188; 五年影响因子:2.197 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: The gene (Bhbgl) encoding a beta-glucosidase from the alkalophilic bacterium Bacillus halodurans C-125 was synthesized chemically via the PCR-based two-step DNA synthesis (PTDS) method and expressed in Escherichia coli. Bhbgl contained an open reading frame (ORF) of 1359 bp encoding a 453-amino acid protein belonging to glycoside hydrolase family 1 (GHF1), and the deduced molecular mass of recombinant Bhbgl (52,488 Da) was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme exhibited a high specific activity with o-nitrophenyl-beta-d-glucopyranoside (oNPGlu) and an apparent K (m) value of 0.32 mM. With oNPGlu as the substrate, Bhbgl displayed pH and temperature optima of similar to 7.0 and 50A degrees C, respectively. The enzyme was relatively stable under alkaline conditions and > 50% activity was retained after incubation at pH 9.5 for 24 h at 4A degrees C. Recombinant Bhbgl activity was inhibited by 5 mM Zn2+, Fe3+, or Cd2+, but was enhanced by 1 mM Mg2+ and other metal ions. Enzyme activity was also stimulated by at least four sugars (sucrose, d-galactose, xylose, glucose) at concentrations ranging from 50 to 800 mM.
分类号: Q93
- 相关文献
作者其他论文 更多>>
-
Nutrient and metabolite characteristics of the husk, bran and millet isolated from the foxtail millet (Setaria italica L.) during polishing
作者:Li, Pengliang;Cai, Xinru;Li, Shaohui;Zhao, Wei;Liu, Junli;Zhang, Xiaodi;Zhang, Aixia;Liu, Jingke;Li, Zengning;Li, Zengning;Guo, Linlin
关键词:Foxtail millet; Husk; Bran; Nutrient; Metabolomics
-
Rumen and hindgut microbiome regulate average daily gain of preweaning Holstein heifer calves in different ways
作者:Xu, Sheng-yang;Feng, Xiao-ran;Zhao, Wei;Bi, Yan-liang;Diao, Qi-yu;Tu, Yan
关键词:Gastrointestinal microbiome; Gastrointestinal metabolome; Host metabolome; Holstein heifer calves; Preweaning ADG
-
The effect of heat moisture treatment times on physicochemical and digestibility properties of adzuki bean, pea, and white kidney bean flours and starches
作者:Li, Shaohui;Li, Pengliang;Zhao, Wei;Zhang, Aixia;Liu, Jingke;Zhang, Liu;Sheng, Qinghai
关键词:Heat moisture treatment; Beans; Physicochemical properties; Digestibility
-
Physicochemical properties of whole grain foxtail millet sourdough and steamed bread quality: Co-fermentation of lactic acid bacteria and yeast in whole grain fermented foods
作者:Li, Shaohui;Yi, Huaxi;Li, Shaohui;Zhang, Aixia;Zhao, Wei;Li, Pengliang;Si, Xingxue;Liu, Jingke
关键词:Sourdough; Physicochemical characterization; Flavor; Foxtail millet steamed bread
-
High-quality genome assembly enables prediction of allele-specific gene expression in hybrid poplar
作者:Shi, Tian-Le;Jia, Kai-Hua;Bao, Yu-Tao;Tian, Xue-Chan;Yan, Xue-Mei;Chen, Zhao-Yang;Li, Zhi-Chao;Zhao, Shi-Wei;Ma, Hai-Yao;Zhao, Ye;An, Xinmin;Mao, Jian-Feng;Jia, Kai-Hua;Nie, Shuai;Nie, Shuai;Nie, Shuai;Li, Xiang;Zhang, Ren-Gang;Guo, Jing;Zhao, Wei;Wang, Xiao-Ru;El-Kassaby, Yousry Aly;Mueller, Niels;van de Peer, Yves;van de Peer, Yves;van de Peer, Yves;van de Peer, Yves;Street, Nathaniel Robert;Mao, Jian-Feng;Porth, Ilga
关键词:
-
Effect of Feeding Level on Growth and Slaughter Performance, and Allometric Growth of Tissues and Organs in Female Growing Saanen Dairy Goats
作者:Huang, Juan;Jiao, Shuai;Fu, Yuze;Zhao, Wei;Diao, Qiyu;Ma, Tao;Zhang, Naifeng
关键词:Saanen dairy goats; growth performance; slaughter performance; organ index; allometric growth
-
The complete degradation of 1,2-dichloroethane in Escherichia coli by metabolic engineering
作者:Deng, Yong-Dong;Zhang, Wen -Hui;Zuo, Zhi-Hao;Zhang, Hao;Xu, Jing;Gao, Jian- Jie;Wang, Bo;Li, Zhen-Jun;Fu, Xiao-Yan;Wang, Li -Juan;Wang, Yu;Tian, Yong-Sheng;Peng, Ri-He;Yao, Quan-Hong;Deng, Yong-Dong;Zhang, Wen -Hui;Zuo, Zhi-Hao;Zhang, Hao;Xu, Jing;Gao, Jian- Jie;Wang, Bo;Li, Zhen-Jun;Fu, Xiao-Yan;Wang, Li -Juan;Wang, Yu;Tian, Yong-Sheng;Peng, Ri-He;Yao, Quan-Hong
关键词:Bioremediation; Engineered bacteria; Environmental pollution; Metabolic engineering; Synthetic biology