Two coiled-coil regions of Xanthomonas oryzae pv. oryzae harpin differ in oligomerization and hypersensitive response induction

文献类型： 外文期刊

第一作者： Ji, Zhaolin

作者： Ji, Zhaolin;Song, Congfeng;Wang, Jinsheng;Lu, Xuzhong

作者机构：

关键词： Xanthomonas oryzae;Hpal;Coiled-coil;Oligomerization;α-helix;Hypersensitive response

期刊名称：AMINO ACIDS （ 影响因子：3.52； 五年影响因子：3.6 ）

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收录情况： SCI

摘要： Hpalxoo (harpin) is a type III secreted protein of the rice blight bacterial pathogen Xanthomonas oryzae pv. oryzae that elicits a hypersensitive response (HR) in nonhost tobacco. Hpal_(Xoo) is predicted to contain two potential coiled-coil (CC) regions, one at the N-terminus with a high probability of formation, and one at the C-terminus with a lower probability of formation. We constructed several CC-equivalent peptides by a chemo-synthetic method, and investigated the structure-function of the predicted Hpal_(Xoo) CC regions, using biophysical and biochemical approaches. Both peptides elicited an HR in tobacco. Mutant versions of the N- and C-terminal peptides that were predicted to disrupt or favor CC formation were generated. The resulting altered HR activity and oligomerization indicated that the N-terminal CC region is essential for eliciting HR, but the C-terminus is not. The results also indicate that a 14-residue fragment (LDQLLCQLISALLQ) within the N-terminal CC region is a minimal and independent functional element for HR-induction in tobacco leaves. We propose that HR-induction requires a specific oligomerization of the CC regions of Hpalxoo-

分类号： Q5

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