Maternal transfer of florfenicol impacts development and disrupts metabolic pathways in F1 offspring zebrafish by destroying mitochondria

文献类型： 外文期刊

第一作者： Zhang, Lin

作者： Zhang, Lin;Qiu, Jing;Li, Yameng;He, Linjuan;Mao, Mingcai;Wang, Tiancai;Pan, Yecan;Li, Zishu;Mu, Xiyan;Qian, Yongzhong;Zhang, Lin;Qiu, Jing;Li, Yameng;He, Linjuan;Mao, Mingcai;Wang, Tiancai;Pan, Yecan;Li, Zishu;Mu, Xiyan;Qian, Yongzhong;Qian, Yongzhong

作者机构：

关键词： Florfenicol; Offspring; Development toxicity; Metabolism; Mitochondria; Reactive oxygen species

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.8； 五年影响因子：6.9 ）

ISSN： 0147-6513

年卷期： 2023 年 252 卷

页码：

收录情况： SCI

摘要： Maternal exposure to antibiotics existing in the environment is a predisposing factor for developmental mal-formation with metabolic disorders in offspring. In this study, female zebrafish (3 months) were exposed to 0.05 mg/L and 0.5 mg/L florfenicol (FF) for 28 days. After pairing and spawning with healthy male fish, F1 embryos were collected and developed to 5 d post-fertilization (dpf) in clear water. And the adverse effects on the F1 generation were examined thoroughly. The fecundity of F0 female fish and the hatchability, mortality, and body length of F1 larvae significantly decreased in the treatment group. Meanwhile, multi-malformation types were found in the exposure group, including delayed yolk sac absorption, lack of swim bladder, and spinal curvature. Metabolomic and transcriptomic results revealed alterations in metabolism with dysregulation in tricarboxylase acid cycle, amino acid metabolism, and disordered lipid metabolism with elevated levels of glycerophospholipid and sphingolipid. Accompanying these metabolic derangements, decreased levels of ATP and disordered oxidative-redox state were observed. These results were consistent with the damaged mitochondrial membrane potential and respiratory chain function, suggesting that the developmental toxicity and perturbed metabolic signaling in the F1 generation were related to the mitochondrial injury after exposing F0 female zebrafish to FF. Our findings highlighted the potential toxicity of FF to offspring generations even though they were not directly exposed to environmental contaminants.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序