The Nonstructural Protein 1 Papain-Like Cysteine Protease Was Necessary for Porcine Reproductive and Respiratory Syndrome Virus Nonstructural Protein 1 to Inhibit Interferon-beta Induction

文献类型: 外文期刊

第一作者: Shi, Xibao

作者: Shi, Xibao;Zhang, Gaiping;Wang, Li;Li, Xuewu;Zhi, Yubao;Wang, Fangyu;Fan, Jianming;Deng, Ruiguang;Fan, Jianming

作者机构:

关键词: nonstructural protein-1;papain-like cysteine protease: 37353-41-6;interferon-beta: IFN-beta;74899-71-1;production;induction;nonstructural protein-1-alpha;nonstructural protein-1-beta;enzyme activity

期刊名称:DNA AND CELL BIOLOGY ( 影响因子:3.311; 五年影响因子:3.557 )

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收录情况: SCI

摘要: Porcine reproductive and respiratory syndrome virus nonstructural protein 1 (nsp1) could be auto-cleaved into nsp1 alpha and nsp1 beta, both of which had the papain-like cysteine protease activities. Previous studies have shown that porcine reproductive and respiratory syndrome virus nsp1 was an interferon (IFN) antagonist. However, the mechanism by which nsp1 inhibited IFN-beta production was unclear. Here, we used site-directed mutagenesis that inactivated the papain-like cysteine protease activities of nsp1 to explore whether the papain-like cysteine protease activities were required for nsp1 to disrupt IFN-beta production. The results showed that mutations that inactivated papain-like cysteine protease activity of nsp1 alpha made nsp1 lose its IFN antagonism activity, whereas mutations that inactivated papain-like cysteine protease activity of nsp1 beta did not influence the IFN antagonism activity of nsp1. In conclusion, our present work indicated that the papain-like cysteine protease activity of nsp1 alpha was necessary for nsp1 to inhibit IFN-beta induction.

分类号: Q343

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