Molecular markers for tracking variation in lipoxygenase activity in wheat breeding

文献类型: 外文期刊

第一作者: Geng, Hongwei

作者: Geng, Hongwei;Zhang, Yan;He, Zhonghu;Xia, Xianchun;Geng, Hongwei;Qu, Yanying;He, Zhonghu;Zhang, Liping;Appels, Rudi

作者机构:

关键词: molecular marker;SSR marker;lipoxygenase: LOX;63551-74-6;EC 1.13.11.12;phenotypic variance;enzyme activity

期刊名称:MOLECULAR BREEDING ( 影响因子:2.589; 五年影响因子:2.75 )

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年卷期:

页码:

收录情况: SCI

摘要: Lipoxygenase (LOX) activity is an important factor determining the color of flour and end-use products of wheat. In the present study, quantitative trait loci (QTL) for LOX activity in common wheat were mapped using 71 doubled haploid (DH) lines derived from a Zhongyou 9507 x CA9632 cross, and SSR markers. Two QTL, QLpx.caas.1AL and QLpx.caas-4B, were identified on chromosomes 1AL and 4B, closely associated with LOX activity. The SSR loci Xwmc312 and Xgwm251 proved to be diagnostic and explained 13.4-25.2% of the phenotypic variance for the 1AL locus and 14.3-27.0% for the 4B locus across four environments. The SSR markers Xgwm251 and Xwmc312 were validated across 198 Chinese wheat cultivars and advanced lines and showed highly significant (P < 0.01) association with LOX activity. We further established a multiplexed PCR with SSR marker combination Xwmc312/Xgwm251 to test these wheat cultivars and advanced lines. The results suggested that the marker combination Xwmc312/Xgwm251 is efficient and reliable for evaluating LOX activity and can be used in marker-assisted selection (MAS) for targeting flour color attributes to noodle and other wheat-based products.

分类号: Q94

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