A Simple Sequence Repeat Marker Linked to the Susceptibility of Apple to Alternaria Blotch Caused by Alternaria alternata Apple Pathotype

文献类型： 外文期刊

第一作者： Zhang, Liyi

作者： Zhang, Liyi;Cong, Peihua;Li, Ying;Zhang, Zhen;Cheng, Zong-Ming;Cheng, Zong-Ming

作者机构：

关键词： genomic DNA;simple sequence repeat markers;primer pairs;fungicideUSA: North America;Nearctic region;Japan: Asia;Palearctic region;South Korea: Asia;Palearctic region;Liaoning: China;Asia;Palearctic region

期刊名称：JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE （ 影响因子：1.144； 五年影响因子：1.617 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Alternaria alternata apple pathotype (previously A. mali) causes alternaria blotch disease of apple (Malus xdomestica), which may result in leaf spots and up to 70% premature leaf drop in serious cases. This disease is of worldwide importance but is most serious in eastern Asia (Japan, Korea, and China) and in parts of the United States. The excessive use of fungicides not only adds cost to apple growers, but also pollutes the environment. In this study, we characterized a 5-year F-1 population from a cross of a resistant cultivar (Huacui) and a susceptible cultivar (Golden Delicious) consisting of 110 individuals along with 14-year-old parent trees (10 each). A field evaluation of disease severity was conducted in 2008 and 2009 under the natural conditions in Liaoning, China (lat. 40 degrees 37' N, long. 120 degrees 44' E). Based on the field data, 110 F-1 plants were divided into five groups. Artificial inoculation was carried out both on the living trees and on the detached leaves in 2009 to ensure that A. alternata apple pathotype was the causative agent. Eighty primer pairs of simple sequence repeat (SSR) were screened against the four genomic DNA pools, respectively, from six highly susceptible F-1 plants, six most resistant F-1 plants, one tree of the seed parent, and the one tree of the pollen parent. One pair of primers (CH05g07) was shown to be linked to the DNA pools of susceptible F-1 and the parent tree, but not to the DNA pools of resistant F-1 and parent trees. This primer pair was then used to screen all individual 110 F-1 progenies and two parent trees. The differentiation of 103 individuals (97.3%) with the marker matched the field disease resistance rating. This marker was further screened with 20 cultivars with known susceptibility or resistance to A. alternata apple pathotype and its linkage to susceptibility was validated. These results suggest that this marker can be used in marker-assisted selection for resistance/susceptibility to alternaria blotch disease in apple.

分类号： S6

  • 相关文献

[1]Frequency of Bt resistance alleles in Helicoverpa armigera in 2007-2009 in the Henan cotton growing region of China. Feng, Hongqiang,Qiu, Feng,Gao, Yulin.

[2]Evaluation of genetic identity and variation in cultivars of Pyrus pyrifolia (Burm.f.) Nakai from China using microsatellite markers. Cao, Y.,Yang, J.,Zhang, S.,Cao, Y.,Tian, L.,Gao, Y..

[3]Long-Term Evaluation of Manure Application on Maize Yield and Nitrogen Use Efficiency in China. Duan, Yinghua,Xu, Minggang,Wang, Bairen,Yang, Xueyun,Huang, Shaomin,Gao, Suduan.

[4]Analysis of variable genomic loci in white spot syndrome virus to predict its origins in Procambarus clarkii crayfish farmed in China. Zeng, Yong,Zeng, Wei,Fei, Rong-Mei,Zeng, Ling-Bing,Wei, Kai-Jin.

[5]Susceptibility of Helicoverpa armigera from different host plants in northern China to Bacillus thuringiensis toxin Cry1Ac. Gao, Yulin,An, Jingjie,Wu, Kongming,Gould, Fred,Blanco, Carlos A..

[6]Comparison of Growth and Physiological Responses to Severe Drought between Two Altitudinal Hippophae rhamnoides Populations. Yang, Yongqing,Yao, Yinan,Yang, Yongqing,Zhang, Xuejiang.

[7]Comparisons of cadmium tolerance and accumulation at seedling stage in wheat varieties grown in different decades in China. Jiang, Dong,Ci, Dunwei,Jiang, Dong,Dai, Tingbo,Cao, Weixing,Liu, Fulai,Ci, Dunwei.

[8]Investigation of the genetic diversity of an invasive whitefly (Bemisia tabaci) in China using both mitochondrial and nuclear DNA markers. Gao, C. S.,Chu, D.,Gao, C. S.,Gao, C. S.,De Barro, P.,Wan, F. H.,Zhang, Y. J..

[9]Salinity tolerance as well as osmotic and ionic regulation in juvenile Chinese sturgeon (Acipenser sinensis Gray, 1835) exposed to different salinities. Zhao, F.,Qu, L.,Zhuang, P.,Zhang, L.,Liu, J.,Zhang, T.,Qu, L..

[10]Isolation and characterization of 40 microsatellite loci for oriental river prawn (Macrobrachium nipponense) and cross-species utility. Fu, Hongtuo,Wu, Yan,Gong, Yongsheng,Jiang, Sufei,Xiong, Yiwei,Qiao, Hui,Li, Fajun,Fu, Hongtuo,Wang, Ning.

[11]Nitrous oxide emissions from irrigated and fertilized spring maize in semi-arid northern China. Liu, Y. T.,Li, Y. E.,Wan, Y. F.,Gao, Q. Z.,Qin, X. B.,Liu, Y. T.,Li, Y. E.,Wan, Y. F.,Gao, Q. Z.,Qin, X. B.,Chen, D. L.,Li, Y..

[12]CRYPTIC INVASION OF THE EXOTIC BEMISIA TABACI BIOTYPE Q OCCURRED WIDESPREAD IN SHANDONG PROVINCE OF CHINA. Chu, Dong,Chu, Dong,Zhang, You Jun,Wan, Fang Hao.

[13]Influence of whole corn feeding on the performance, digestive tract development, and nutrient retention of geese. Wang, Z. Y.,Zhang, K. N.,Lu, J.,Kong, X. L.,Wang, Z. Y.,Yang, H. M.,Zou, J. M..

[14]Bioremediation efficiencies of Gracilaria verrucosa cultivated in an enclosed sea area of Hangzhou Bay, China. Huo, Yuan Zi,Xu, Shan Nan,Wang, Yang Yang,Zhang, Jian Heng,Zhang, Yin Jiang,Wu, Wei Ning,He, Pei Min,Chen, Ya Qu.

[15]Development of 12 polymorphic microsatellite markers in Coilia ectenes Jordan and Seale, 1905 (Clupeiformes: Engraulidae) and cross-species amplification in Coilia mystus Linnaeus, 1758. Ma, Chunyan,Cheng, Qiqun,Zhang, Qingyi.

[16]Sources of Resistance to Race 2WF Powdery Mildew in U.S. Watermelon Plant Introductions. Zhang, Haiying,Guo, Shaogui,Gong, Guoyi,Ren, Yi,Xu, Yong,Davis, Angela R..

[17]Dynamics of Soil and Grain Micronutrients as Affected by Long-Term Fertilization in an Aquic Inceptisol. Li Ben-Yin,Huang Shao-Min,Shen A-Lin,Li Ben-Yin,Shen A-Lin,Xu Jian-Ming,Wei Ming-Bao,Zhang, H. L.,Ruan Xin-Ling.

[18]The dynamics of dissolved organic N in the fumigated soils. Li, Guitong,Lin, Qimei,Zhao, Xiaorong,Cao, Aocheng.

[19]Phosphorus efficiency in a long-term wheat-rice cropping system in China. Tang, X.,Tang, X.,Ma, Y.,Shi, X.,Hao, X..

[20]Relationship between nitrogen uptake and use efficiency of winter wheat grown in the North China Plain. Tong, Y. P.,Wang, R. F.,An, D. G.,Hu, C. S.,Zhang, Y. M.,Jia, Y. G.,Li, L. H.,Wang, R. F..

作者其他论文 更多>>

微信小程序