Warfarin resistance test and polymorphism screening in the VKORC1 gene in Rattus flavipectus

文献类型： 外文期刊

第一作者： Yue, Ling-Fen

作者： Yue, Ling-Fen;Wang, Da-Wei;Liu, Xiao-Hui;Feng, Zhi-Yong;Yao, Dan-Dan;Gao, Zhi-Xiang

作者机构：

关键词： Rattus flavipectus;Warfarin-resistance test;VKORC1;SNP

期刊名称：JOURNAL OF PEST SCIENCE （ 影响因子：5.918； 五年影响因子：5.955 ）

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收录情况： SCI

摘要： The buff-breasted rat (Rattus flavipectus) is a major agricultural pest across China. Warfarin-resistant animals have been found in several major provinces in China, and are hampering effective control. Molecular mechanisms underlying resistance to anticoagulant rodenticides have been determined for other species, but genetic information regarding resistance in R. flavipectus remains unknown. The vitamin K 2,3-epoxide reductase complex subunit 1 (VKORC1) encoded by VKORC1 gene is the molecular targetof coumarin anticoagulants, and amino acid substitutions in VKORC1 coding-regions have been reported as one of the supposed mechanisms of warfarin resistance. Here, lethal feeding test in R. flavipectus (n — 36) was conducted in Zhanjiang, China. Fouranimals (11%) survived the test period of 25 days and were identified as warfarin resistance. Polymorphism across the whole genome DNA sequence of the VKORC1 gene was screened out and compared with resistant and non-resistant rats. A total of nine singlenucleotide polymorphisms (SNPs) were identified including seven SNPs in introns and two SNPs in exons, and the SNP (2317A > G) located in exon 3 led to the amino acid substitution (Tyrl39Cys) in VKORC1 protein. Based on the characteristics of Tyrl39Cysmutation of VKORC1 in humans or rats and its relationship with warfarin-resistance, Tyrl39Cys mutation may be one mutation responsible for anticoagulant resistance in R. flavipectus. Given the low numbers of resistant rats in our feeding test, wider surveillance, tests of resistance development in a larger wild population and further researches on the genetic mechanisms of anticoagulant resistance in R. flavipectus are necessary.

分类号： S4

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