Induction of meiotic gynogenesis in paddlefish (Polyodon spathula) and its confirmation using microsatellite markers

文献类型： 外文期刊

第一作者： Zou, Y. C.

作者： Zou, Y. C.;Zou, Y. C.;Wei, Q. W.;Pan, G. B.;Wei, Q. W.;Pan, G. B.

作者机构：

关键词： microsatellite marker;UV irradiation;maternal inheritance;meiotic gynogenesis;diploid gynogenesis;gynogenetic progeny;gynogenesis induction;haploid control

期刊名称：JOURNAL OF APPLIED ICHTHYOLOGY （ 影响因子：0.892； 五年影响因子：0.956 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A protocol for gynogenesis of P. spathula was developed in order to produce inbred lines and to develop a technique to preserve the endangered Chinese paddlefish (Psephurus gladius). Diploid gynogenesis was induced in P. spathula using ultraviolet (UV)-irradiated Amur sturgeon (Acipenser schrenckii) sperm for 4.5-min, 5-min or 5.5-min and subsequent heat shock treatment. UV irradiation of sperm for 5 min at a UV intensity (254 nm) of 863 mu w cm(-2) was the optimum dose to achieve diploid gynogenesis on the basis of observations on hatching rate of eggs. Three microsatellite loci were used to monitor exclusive maternal inheritance of gynogenetic progenies. The results showed all maternal genome among offspring with no paternal genome. The cytogenetic analysis showed that meiotic gynogenetic diploids possessed 120 chromosomes in metaphase plates, while haploid control groups N1-3 possessed 60 chromosomes.

分类号： S9

  • 相关文献

[1]Fast development of genetically uniform strains by gynogenesis in Japanese flounder (Paralichthys olivaceus). Jiang, Hongbo,Bao, Jie,Jiang, Hongbo,Liu, Haijin,Wang, Guixing,Zhang, Xiaoyan,Jiang, Li.

[2]Production of gynogenetic diploid Polyodon spathula using fertilization with irradiated sperm followed by spontaneous diploidization and distant hybridization caused by heat shock. Zou, Y. C.,Wei, Q. W.,Zou, Y. C.,Wei, Q. W.,Zeng, L. B.,Li, P.,Wei, Q. W.,Zeng, L. B.,Li, P.,Li, P..

[3]Genetic variability among the chloroplast genomes of sugarcane (Saccharum spp) and its wild progenitor species Saccharum spontaneum L.. Lu, X.,Zhou, H.,Pan, Y. -B.. 2014

[4]Studies on insect resistance of Bt transplastomic plants and the phenotype of their progenies. Zhang, ZL,Chen, X,Qian, KX,Shen, GF. 1999

[5]Chloroplast subspecies-specific SNP detection and its maternal inheritance in Brassica oleracea L. by using a dCAPS marker. Zhang, Yangyong,Fang, Zhiyuan,Wang, Qingbiao,Liu, Yumei,Yang, Limei,Zhuang, Mu,Sun, Peitian.

[6]Characterization of eleven monosomic alien addition lines added from Gossypium anomalum to Gossypium hirsutum using improved GISH and SSR markers. Wang, Xiaoxiao,Wang, Yingying,Wang, Chen,Chen, Yu,Chen, Yu,Feng, Shouli,Zhao, Ting,Zhou, Baoliang,Chen, Yu. 2016

[7]Inducement and identification of chromosome introgression and translocation of Gossypium australe on Gossypium hirsutum. Wang, Yingying,Feng, Shouli,Li, Sai,Tang, Dong,Chen, Yu,Chen, Yu,Zhou, Baoliang,Chen, Yu,Chen, Yu. 2018

[8]Discovery and characterization of a first set of polymorphic microsatellite markers in Siganus oramin. Liu, Y. -X.,Ma, C. -Y.,Ma, H. -Y.,Feng, C. -L.,Li, S. -J.,Ma, L. -B.,Liu, Y. -X.. 2015

[9]Isolation of novel microsatellite markers from Paralichthys lethostigma (Paralichthyidae) and their cross-species application in Pleuronectiformes. Xu, Y. -J.,Liao, M. -J.,Wang, Y. -G.,Liu, Z. -C.,Qin, P.,Zhang, Z.,Rong, X. -J.,Liu, X. -Z.. 2013

[10]Germplasm of breeding Pseudosciaena crocea as revealed by microsatellite markers. Chang Yumei,Ding Lei,Liang Liqun,Chang Yumei,He Jianguo,Ding Lei,Li Mingyun,Xue Liangyi,Liang Liqun,Lei Qingquan. 2008

[11]Evaluation of the genetic diversity and population structure of five indigenous and one introduced Chinese goose breeds using microsatellite markers. Li, Jinjun,Yuan, Qingyuan,Shen, Junda,Tao, Zhengrong,Li, Guoqing,Tian, Yong,Wang, Deqian,Chen, Li,Lui, Lizhi. 2012

[12]Identification of twenty novel polymorphic microsatellite DNA markers from transcripts of the pearl oyster Pinctada fucata using next-generation sequencing approach. Fan, Sigang,Wang, Jingxuan,Huang, Guiju,Liu, Baosuo,Yu, Dahui,Wang, Jingxuan. 2015

[13]Isolation and characterization of novel polymorphic microsatellite markers for Lutjanus erythropterus. Lin, L.,Li, C. H.,Xu, S. N.,Liu, Y.,Xiao, Y. Y.,Lin, L.,Li, C. H.,Xu, S. N.,Liu, Y.,Xiao, Y. Y.. 2015

[14]Molecular tagging of the yellow rust resistance gene Yr10 in common wheat, PI178383 (Triticum aestivum L.). Wang, LF,Ma, JX,Zhou, RH,Wang, XM,Jia, JZ. 2002

[15]Genetic characterization of three breeds of high royal jelly producing honeybee (Apis mellifera ligustica) in China. Yin, Ling,Ji, Ting,Chen, Guohong,Peng, Wenjun. 2011

[16]Genetic variability of half-smooth tongue sole Cynoglossus semilaevis populations using microsatellite markers. Liu Shuaishuai,Ye Naihao,Liu Yunguo,Liu Yunguo,Liu Shuaishuai,Guo Yinghui,Li Junfeng. 2011

[17]Isolation and characterization of microsatellite markers for Glytothorax zanaensis. Liu, Shao-Ping,Yuan, Xi-Ping,Wang, Deng-Qiang,Yue, Xing-Jian,Zhang, Yan,Wang, Ke,Duan, Xin-Bin,Chen, Da-Qing. 2009

[18]Isolation and characterization of twenty microsatellite markers for the endangered fish Alburnus chalcoides. Geng Longwu,Jiang Haifeng,Tong Guangxiang,Lu Cuiyun,Li Chao,Xu Wei. 2013

[19]Isolation and characterization of polymorphic microsatellite markers in the yellow catfish, Pelteobagrus fulvidraco. Hu, Guang-Fu,Liang, Hong-Wei,Li, Zhong,Wang, Chang-Zhong,Wu, Qin-Chao,Liu, Xiang-Jiang,Luo, Xiang-Zhong,Zou, Gui-Wei. 2009

[20]Development of microsatellite markers and their utilization in genetic diversity analysis of cultivated and wild populations of the mud carp (Cirrhina molitorella). Yang, Cheng,Sun, Xiaowen,Yang, Cheng,Zhu, Xinping. 2008

作者其他论文 更多>>

微信小程序