Antioxidant supplementation, effect on post-thaw spermatozoan function in three sturgeon species

文献类型： 外文期刊

第一作者： Li, P.

作者： Li, P.;Xi, M. D.;Du, H.;Qiao, X. M.;Liu, Z. G.;Wei, Q. W.;Li, P.;Xi, M. D.;Du, H.;Qiao, X. M.;Liu, Z. G.;Wei, Q. W.;Li, P.

作者机构：

关键词： antioxidant; cryopreservation; sperm quality; spermatozoa; sturgeon

期刊名称：REPRODUCTION IN DOMESTIC ANIMALS （ 影响因子：2.005； 五年影响因子：2.154 ）

ISSN： 0936-6768

年卷期： 2018 年 53 卷 2 期

页码：

收录情况： SCI

摘要： Contents High levels of reactive oxygen species (ROS), which may be associated with reduced sperm quality, can be detected during cryopreservation of sperm of some species. Our objective was to investigate whether the addition of antioxidants to cryopreservation extenders influenced post-thaw sperm characteristics and fertility in Acipenser dabryanus, Acipenser sinensis and Acipenser baerii. Prior to freezing, sperm samples were diluted with a base extender as control or in extender supplemented with catalase (CAT), glutathione (GSH), cysteine (NAC), ascorbic acid (VC) or their paired combinations. Protective concentrations of CAT, GSH and VC in the three species were 25U/ml, 0.25-0.5mg/ml and 0.5mg/ml, respectively. Cysteine showed no protective effect against ROS. The addition of CAT, GSH and VC positively affected either acrosome or membrane integrity of post-thawed sperm in the three species, as well as spermatozoan motility in A.sinensis. The combination of antioxidants did not show a positive synergistic effect. This study suggested that the use of antioxidants in the cryopreservation of sturgeon sperm has potential to decrease intracellular ROS, and consequently preserve acrosome and membrane integrity, as well as spermatozoan motility.

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