Expression of the hIGF-I gene driven by the Fhx/P25 promoter in the silk glands of germline silkworm and transformed BmN cells

文献类型： 外文期刊

第一作者： Li, Yanmei

作者： Li, Yanmei;Cao, Guangli;Wang, Yang;Xue, Renyu;Gong, Chengliang;Cao, Guangli;Xue, Renyu;Gong, Chengliang;Zhou, Wenlin;Gong, Chengliang

作者机构：

关键词： BmN cell;Bombyx mori;Fhx/P25 promoter;hIGF-I;Silk gland;Transgenic silkworm

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）

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收录情况： SCI

摘要： The expression of the human insulin-like growth factor (hIGF-I) gene driven by the Fhx/P25 promoter in the silk glands of transgenic silkworms (Bombyx mori) and in transformed silkworm cells, was achieved using BmN cells transfected with a piggyBac vector, pigA3GFP-Fhx/P25-hIGF-ie-neo containing a neomycin-resistance gene (neo), a green fluorescent protein gene (gfp), an hIGF-I gene, and a helper plasmid containing the piggyBac transposase sequence under the control of the B. mori actin 3 (A3) promoter. We selected stably transformed BmN cells expressing hIGF-I using the antibiotic G418. The expression level of hIGF-I was about 450pg in 310 super(6) cells, determined by ELISA. The piggyBac vector was transferred into the silkworm eggs using sperm-mediated gene transfer. The expression level of hIGF-I per gram fresh posterior silk glands of G4 transgenic silkworms was approx. 150ng.

分类号： Q7

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