Identification of serine acetyltransferase (SAT) gene family in peach (Prunus persica) and study on the function of PpSAT1 gene regulating adventitious root formation

文献类型： 外文期刊

第一作者： Hao, Lanlan

作者： Hao, Lanlan;Zhang, Fan;Zhang, Xuebing;Yang, Yang;Wang, Hong;Hao, Lanlan;Wang, Hong

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关键词： Prunus persica; SAT gene family; Gene cloning; Adventitious root; Genetic transformation

期刊名称：CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE （ 影响因子：5.2； 五年影响因子：6.1 ）

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年卷期： 2025 年 12 卷 1 期

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收录情况： SCI

摘要： BackgroundSerine acetyltransferase (SAT), an enzyme that catalyzes the formation of O-acetyl-serine (OAS), is integral to sulfur assimilation, cysteine (Cys) synthesis, and adventitious root development. However, it remains unclear how the SAT gene in Prunus persica regulates adventitious root (AR) formation.MethodsBased on transcriptome data and SAT gene family identification, the physicochemical properties, evolutionary relationships, and cis-acting elements of the family genes were analyzed. Subsequently, the PpSAT1 gene was transformed into Prunus domestica and Arabidopsis thaliana by agrobacterium-mediated method to obtain the transgenic material, and its role in AR formation was characterized by a series of rooting index and enzyme activity experiments.ResultsIn this study, based on transcriptome data, the cysteine metabolism pathway was significantly enriched during P. persica AR growth. After combining the FPKM value of transcriptome data with real-time fluorescence quantitative qRT-PCR, it was found that SAT1/4 showed high expression level, which may be a key gene in peach advection root growth. Based on this, SAT family members were identified from P. persica, and further shown by qRT-PCR, PpSAT1 gene exhibits a notable expression response during AR formation. Therefore, the PpSAT1 (Prupe.4G239400.1) gene was cloned from P. persica and performed genetic transformation on a related P. domestica as well as A. thaliana. The transgenic P. domestica and A. thaliana displayed more robust growth, and more developed root system compared to wild-type counterparts. In addition, peroxidase (POD) and superoxide dismutase (SOD) activities were also substantially elevated.ConclusionsIn summary, these findings suggest that PpSAT1 gene can facilitate AR development.

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