Identification of an Enterococcus faecium strain isolated from raw bovine milk co-harbouring the oxazolidinone resistance genes optrA and poxtA in China

文献类型： 外文期刊

第一作者： Hou, Jie

作者： Hou, Jie;Xu, Qiu;Zhou, Longyu;Chai, Jiyun;Lin, Longhua;Ma, Caiping;Zhu, Yao;Zhang, Wanjiang

作者机构：

关键词： Oxazolidinone; optrA; poxtA; Enterococcus faecium; Raw bovine milk

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.3； 五年影响因子：3.5 ）

ISSN： 0378-1135

年卷期： 2024 年 293 卷

页码：

收录情况： SCI

摘要： Oxazolidinones are potent antimicrobial agents used to treat human infections caused by multidrug-resistant Gram-positive bacteria. The growing resistance to oxazolidinones poses a significant threat to public health. In August 2021, a linezolid-resistant Enterococcus faecium BN83 was isolated from a raw milk sample of cow in Inner Mongolia, China. This isolate exhibited a multidrug resistance phenotype and was resistant to most of drugs tested including linezolid and tedizolid. PCR detection showed that two mobile oxazolidinones resistance genes, optrA and poxtA , were present in this isolate. Whole genome sequencing analysis revealed that the genes optrA and poxtA were located on two different plasmids, designated as pBN83 -1 and pBN83 -2, belonging to RepA_N and Inc18 families respectively. Genetic context analysis suggested that optrA gene on plasmid pBN83 -1 was located in transposon Tn 6261 initially found in E. faecalis. Comprehensive analysis revealed that Tn 6261 act as an important horizontal transmission vector for the spread of optrA in E. faecium. Additionally, poxtA -bearing pBN83 -2 displayed high similarity to numerous plasmids from Enterococcus of different origin and pBN83 -2-like plasmid represented a key mobile genetic element involved in movement of poxtA in enterococcal species . The presence of optrA - and poxtA -carrying E. faecium in raw bovine milk represents a public health concern and active surveillance is urgently warranted to investigate the prevalence of oxazolidinone resistance genes in animalderived food products.

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