Occurrence and Detection of Carbendazim Resistance in Botryosphaeria dothidea from Apple Orchards in China

文献类型： 外文期刊

第一作者： Wang, Li

作者： Wang, Li;Tu, Hongtao;Hou, Hui;Zhou, Zengqiang;Yuan, Hongbo;Gu, Qinsheng;Wang, Li;Luo, Chaoxi

作者机构：

关键词： apple white rot; Botryosphaeria dothidea; carbendazim resistance; E198A mutation; loop-mediated isothermal amplification (LAMP)

期刊名称：PLANT DISEASE （ 影响因子：4.614； 五年影响因子：5.33 ）

ISSN： 0191-2917

年卷期： 2022 年 106 卷 1 期

页码：

收录情况： SCI

摘要： Botryosphaeria dothidea causes white rot, which is among the most devastating diseases affecting apple crops globally. In this study, we assessed B. dothidea resistance to carbendazim by collecting samples from warts on the infected branches of apple trees or from fruits exhibiting evidence of white rot. All samples were collected from different orchards in nine provinces of China in 2018 and 2019. In total, 440 B. dothidea isolates were evaluated, of which 19 isolates from three provinces were found to exhibit carbendazim resistance. We additionally explored the fitness and resistance stability of these isolates, revealing that they were no less fit than carbendazim-sensitive isolates in terms of pathogenicity, sporulation, and mycelial growth and that the observed carbendazim resistance was stable. Sequencing of the beta-tubulin gene in carbendazim-resistant isolates showed the presence of a substitution at codon 198 (GAG to GCG) that results in an alanine substitution in place of glutamic acid (E198A) in all 19 resistant isolates. A loop-mediated isothermal amplification (LAMP) method was then developed to rapidly and specifically identify this E198A mutation. This LAMP method offers value as a tool for rapidly detecting carbendazim-resistant isolates bearing this E198A mutation and can thus be used for the widespread monitoring of apple crops to detect and control the development of such resistance.

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