Transcriptomic analyses reveal potential mechanisms of premature senescence in hexaploid Populus

文献类型： 外文期刊

第一作者： Zeng, Qingqing

作者： Zeng, Qingqing;Zeng, Qingqing;Ren, Yongyu;Kang, Xiangyang;Zeng, Qingqing;Ren, Yongyu;Kang, Xiangyang;Zeng, Qingqing;Ren, Yongyu;Wang, Peiqi

作者机构：

关键词： Populus; Chromosome doubling; Hexaploid; Premature senescence; Transcriptome

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN： 0167-6857

年卷期： 2021 年 147 卷 2 期

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收录情况： SCI

摘要： The important role of polyploidy in plant evolution is widely recognized. However, many questions concerning how polyploidy affects the plant phenotype remain unexplored. To elucidate the phenotypic and molecular effects of polyploidization, we obtained hexaploids by applying colchicine to a triploid clone (Populus tomentosa x P. bolleana) x (P. alba x P. glandulosa). The highest hexaploid induction rate (3.57%) resulted from a 3-day pre-culture treatment followed by treatment with colchicine by immersing the explant in 100 mg l(-1) colchicine for 96 h. Reduction of photosynthesis and chloroplast degradation was observed in leaves of the hexaploid Populus which is an indication of early senescence. To investigate the gene expression underlying how polyploidization causes premature senescence, we compared the transcriptomes of the seventeenth leaf between hexaploid and triploid Populus; the leaf position was determined on the basis of preliminary experiments. 16,403 differentially expressed genes (DEGs) were identified between the hexaploids and the triploids. Based on the transcription information, several genes involved in the circadian rhythm and redox status may play important roles in premature senescence caused by polyploidization. Additionally, the MYB, AP2/EREBP, bHLH, NAC and WRKY transcription factors (TFs) may be more important than other TFs in the premature senescence of hexaploid Populus. The transcriptome results were consistent with those of quantitative real-time polymerase chain reaction (qRT-PCR), implying credibility. This study provides insights into the molecular mechanisms of the effects of polyploidization on phenotypic variation, which may be useful for the genetic improvement of polyploid breeding.

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