文献类型: 外文期刊
第一作者: Zhang, Jinpeng
作者: Zhang, Jinpeng;Zheng, Jun;Wang, Guoying;Zhu, Yun;Zhu, Yun;Fu, Junjie;Liu, Tingsong;Jia, Zhiwei;Wang, Jiashi;Jin, Ying;Lian, Yun;Wang, Meng;Hou, Wei;Zhu, Yun;Fu, Junjie;Liu, Tingsong;Jia, Zhiwei;Wang, Jiashi;Jin, Ying;Lian, Yun;Wang, Meng;Hou, Wei
作者机构:
关键词: Maize;Ear;Development;Microarray;Loop design;Auxin
期刊名称:PLANT MOLECULAR BIOLOGY ( 影响因子:4.076; 五年影响因子:4.89 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: In order to unravel the molecular mechanism of maize ear development, a microarray containing similar to 56,000 probes was used to monitor the gene expression profiles of ears at four developmental stages. The results showed that 2,794 genes, accounting for 5.0% of the total probes, changed significantly during ear development. Among the 2,794 genes, 1,844 genes differentially expressed during the spikelet differentiation phase, 836 genes during the floret primordium differentiation phase and 645 genes during the floret organ differentiation phase. Hierarchical clustering revealed that the differentially expressed genes had 9 major expression patterns. Based on Mips Functional Catalogue, 684 differentially expressed genes were grouped into at least one functional category, including metabolism (30.4%), protein related function (29.2%), biogenesis of cellular components (15.4%) and transcription (13.7%). The analysis revealed that the auxin signaling pathway play an important role in ear development. Moreover, regulation of some transcription factors may play a key role during ear development. RT-PCR and in situ hybridization for some selected genes validated our microarray data and supplied additional information on ear developmental processes.
分类号: Q946
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