Isolation and characterization of polymorphic microsatellite DNA markers in the rock bream (Oplegnathus fasciatus)

文献类型： 外文期刊

第一作者： Xu, Tianjun

作者： Xu, Tianjun;Shao, Changwei;Liao, Xiaolin;Ji, Xiangshan;Chen, Songlin;Xu, Tianjun

作者机构：

关键词： Oplegnathus fasciatus;Enriched genomic library;Microsatellites

期刊名称：CONSERVATION GENETICS （ 影响因子：2.538； 五年影响因子：2.32 ）

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收录情况： SCI

摘要： From a (GT)(13)-enriched genomic library of Oplegnathus fasciatus, 14 polymorphic microsatellite were isolated and characterized in a test population with alleles ranging from two to nine, the observed and expected heterozygosities from 0.0000 to 1.0000, and from 0.1726 to 0.8507, respectively. Five loci deviated from the Hardy-Weinberg equilibrium, and linkage disequilibrium between two loci was significant. Two loci were also polymorphic in Pagrosomus major assessed for cross-species amplification. These polymorphic microsatellites will be useful for genetic diversity analysis and molecule-assisted breeding for O. fasciatus.

分类号： Q3

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