Isolation and charaterization of polymorphic microsatellite loci from so-iuy mullet (Mugil soiuy Basilewsky 1855)

文献类型： 外文期刊

第一作者： Xu, Genbo

作者： Xu, Genbo;Shao, Changwei;Liao, Xiaolin;Tian, Yongsheng;Chen, Songlin;Xu, Genbo

作者机构：

关键词： Microsatellite;So-iuy mullet;Mugil soiuy Basilewsky;Enriched genomic library

期刊名称：CONSERVATION GENETICS （ 影响因子：2.538； 五年影响因子：2.32 ）

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年卷期：

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收录情况： SCI

摘要： The first set of polymorphic microsatellite markers were developed from so-iuy mullet (Mugil soiuy Basilewsky 1855). From a (GT)n-enriched genomic library, 53 microsatellites were selected for designing microsatellite primers, of which 36 gave working primer pairs. Ten of these loci were polymorphic in a test population of 24 individuals with alleles ranging from 3 to 9, and observed and expected heterozygosities from 0.2083 to 0.9167 and from 0.2651 to 0.8812, respectively. No significant linkage disequilibrium between pairs of loci was found, but two loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These polymorphic microsatellite loci should provide sufficient level of genetic diversity to investigate the fine-scale population structure and evaluate the breeding strategy in Mullet.

分类号： Q3

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