New polymorphic microsatellite markers for the summer flounder, Paralichthys dentatus
文献类型: 外文期刊
第一作者: Shao, Changwei
作者: Shao, Changwei;Xu, Genbo;Wang, Lei;Liao, Xiaolin;Tian, Yongsheng;Chen, Songlin;Shao, Changwei;Xu, Genbo;Wang, Lei
作者机构:
关键词: Paralichthys dentatus;Enriched genomic library;Microsatellites;Population structure
期刊名称:CONSERVATION GENETICS ( 影响因子:2.538; 五年影响因子:2.32 )
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收录情况: SCI
摘要: Given the ecological and commercial importance of the summer flounder (Paralichthys dentatus), there is a surprising paucity of information on the molecular genetics of this species. Some studies published to date are concentrated on the reproduction biology. To address this shortcoming, a microsatellite-enriched genomic DNA library of P. dentatus was generated and screened by sequencing. Twelve dinucleotide microsatellite loci were characterized by genotyping 24 samples. The observed number of alleles ranged from three to thirteen with an average of 8.25, while the effective number of alleles ranged from 2.21 to 8.28 with an average of 5.06. The observed and expected heterozygosities ranged from 0.0833 to 0.9583 and from 0.5594 to 0.8980, respectively. Significant deviations from Hardy-Weinberg expectations were detected at three loci and linkage disequilibrium between two loci was significant after applying Bonferroni correction. In cross-species amplification, three species showed at least two polymorphic loci. The 12 highly polymorphic microsatellite markers represent a powerful molecular tool, which will allow for detailed population genetic analyses on this important marine fish.
分类号: Q3
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