Establishment of Novel and Efficient Methods for Investigating Sexual Reproduction in Magnaporthe oryzae

文献类型： 外文期刊

第一作者： Cai, Yingying

作者： Cai, Yingying;Wang, Jing;Noman, Muhammad;Hao, Zhongna;Zhang, Zhen;Qiu, Haiping;Chai, Rongyao;Wang, Yanli;Wang, Jiaoyu;Lin, Fucheng;Noman, Muhammad

作者机构：

关键词： conidia mixed mating (CMM); hyphal segments mixed mating (HMM); rice blast; sexual reproduction

期刊名称：JOURNAL OF FUNGI （ 影响因子：4.0； 五年影响因子：4.5 ）

ISSN：

年卷期： 2025 年 11 卷 8 期

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收录情况： SCI

摘要： Rice blast, caused by Magnaporthe oryzae, significantly threatens global rice production. Disease control is complicated by the pathogen's high genetic diversity, which is driven by heterothallic recombination between opposite mating types that underlies variation. However, mechanisms governing sexual reproduction in this fungus remain poorly characterized, largely due to the absence of reliable methods for scalable ascospore progeny production. In this study, we established two novel mating methods, namely Conidial Mixing Mating (CMM) and Hyphal Segments Mixed Mating (HMM). Both methods employed optimized suspensions (5 x 104 conidia/mL or equivalent hyphal density) mixed at 1:1 ratios, incubated under standardized conditions: 20 degrees C with a 12 h/12 h photoperiod. We characterized perithecia, asci, and ascospore morphology using fluorescence microscopy, paraffin sectioning, cryo-scanning electron microscopy, and transmission electron microscopy. Furthermore, both methods enabled phenotypic characterization of sexual reproduction-deficient mutants, including Delta Mopmk1 and Delta Moopy2. In conclusion, we established two efficient methods for investigating M. oryzae sexual reproduction, providing foundational tools to advance studies of sexual mechanisms, pathogenicity evolution, and genetic variation.

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