Bacillus amyloliquefaciens XJ-BV2007 produces lipopetides that significantly inhibit Alternaria alternata and tenuazonic acid accumulation in processing tomatoes

文献类型： 外文期刊

第一作者： Dai, Yingying

作者： Dai, Yingying;Dai, Yingying;Fan, Yingying;Liu, Fengjuan;Wang, Yan;Shen, Tingting;Wang, Peicheng;Zhang, Yiwen;He, Dan;Wu, Aibo;Wang, Cheng

作者机构：

关键词： Bacillus amyloliquequebilis XJ-BV2007; Lipopeptides; Fermentation conditions; Black spot disease; Tenuazonic acid; Tomato

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.5； 五年影响因子：3.6 ）

ISSN： 0882-4010

年卷期： 2025 年 205 卷

页码：

收录情况： SCI

摘要： Black spot disease in tomatoes, caused by Alternaria sp, results in the contamination of the tomatoes with Alternaria mycotoxins, especially tenuazonic acid (TeA). In this regard, TeA accumulation in processing tomatoes and their derived food products represent a serious health hazard. In this regard, our previous study provided evidence that lipopeptides produced by Bacillus amyloliquefaciens XJ-BV2007 effectively inhibit A. alternata and TeA accumulation. In the present study, we significantly increased the production of lipopeptides by optimizing the composition of the culture medium and the fermentation conditions utilizing single-experiments and response surface methodology. The optimal medium (6.0 g/L maltose, 9.0 g/L peptone, and 5.0 g/L yeast extract) and fermentation protocol (4.5 % starter inoculation volume and 44 h of fermentation at 33 degrees C) increased the yield of lipopeptides by 111 %. Spraying field-planted tomato plants with lipopeptides at 10day intervals decreased the incidence of black spot disease in tomato fruit and the level of TeA mycotoxin. Notably, the expression level of AaTAS1, which encodes TeA, was substantially downregulated in diseased tomatoes. A molecular docking model indicated that the lipopeptide fengycin has a strong binding potential with TeA. Results of the study provide a foundation for further exploring the use of B. amyloliquefaciens XJ-BV2007 and/or its lipopeptides as biopesticides for the control of black spot disease and prevention of TeA contamination in processing tomato products.

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