Identification of differentially expressed genes in Tetrahmena thermophila in response to dichlorodiphenyltrichloroethane (DDT) by suppression subtractive hybridization

文献类型: 外文期刊

第一作者: Miao, W

作者: Miao, W;Yu, T;Orias, E;Wan, ML;Fu, CJ

作者机构:

关键词: STEROL CARRIER PROTEIN-2;TETRAHYMENA-THERMOPHILA;CAENORHABDITIS-ELEGANS;CHOLESTEROL;LIVER;PCR

期刊名称:ENVIRONMENTAL MICROBIOLOGY ( 影响因子:5.491; 五年影响因子:6.438 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The insecticide dichlorodiphenyltrichloroethane (DDT) is persistent in the environment, and continues to cause health problems. Tetrahymena has potential as a model organism for assaying low levels of DDT and for analysing the mechanisms of its toxicity. We constructed the suppression subtractive hybridization library of T thermophila exposed to DDT, and screened out 90 Expressed Sequence Tags whose expressions were significantly up- or downregulated with DDT treatment. From this, a series of important genes related to the DDT metabolism and detoxification were discovered, such as P450 gene, glutathione S-transferase gene and sterol carrier protein 2 gene. Furthermore, their expressions under different concentrations of DDT treatment were detected by real-time fluorescent quantitative PCR. The results show that Tetrahymena is a relevant and useful model organism for detecting DDT in the environment and for discovering biomarkers that can be used to develop specific bio-reporters at the molecular and genomic levels.

分类号: Q93

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