Dual DNAzyme-MoS2/GDY Catalytic Assembly Enables Smartphone-Based Multiplex Detection of Sugarcane Pokkah Boeng Pathogens at Sub-Femtomolar Levels

文献类型： 外文期刊

第一作者： Fu, Bingtao

作者： Fu, Bingtao;Che, Rongshuai;Feng, Defen;Yan, Jun;Huang, Ke-Jing;Tan, Xuecai;Wang, Zeping;Ya, Yu

作者机构：

期刊名称：ANALYTICAL CHEMISTRY （ 影响因子：6.7； 五年影响因子：6.6 ）

ISSN： 0003-2700

年卷期： 2025 年 97 卷 34 期

页码：

收录情况： SCI

摘要： Rapid on-site detection of sugarcane pokkah boeng disease caused by Fusarium pathogens remains challenging due to the lack of portable platforms combining high sensitivity and multiplexing capability. Here, we present a self-powered biosensor integrating a dual DNAzyme-driven catalytic system with a MoS2/graphdiyne (GDY) nanohybrid-modified biofuel cell (EBFC) for simultaneous detection of Fusarium sacchari and Fusarium verticillioides. The key innovation lies in the windmill-shaped dual DNAzyme structure that enables Mn2+/Mg2+-dependent target recycling, synergistically coupled with the hybridization chain reaction (HCR) and triplex catalytic hairpin assembly (TCHA) for exponential signal amplification. The MoS2/GDY nanohybrid provides an ideal conductive substrate with 3.8-fold higher DNA loading capacity than pristine MoS2, while the integration of a charge-storage capacitor boosts detection sensitivity by 10.4- and 9.8-fold compared with conventional EBFCs through transient current amplification. The smartphone-coupled system achieves unprecedented detection limits of 21.3 aM (F. sacchari) and 54.3 aM (F. verticillioides) with a dynamic range spanning 5 orders of magnitude (0.1 fM-10 nM), demonstrating excellent specificity against non-target pathogens (more than 95% signal discrimination). This smartphone-integrated biosensor represents a field-ready diagnostic tool for rapid on-site screening of sugarcane fungal pathogens, offering a transformative approach to mitigate crop losses through early disease intervention and precision agriculture management.

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