Polylactic Glycolic Acid-Mediated Delivery of Plectasin Derivative NZ2114 in Staphylococcus epidermidis Biofilms

文献类型： 外文期刊

第一作者： Ma, Xuanxuan

作者： Ma, Xuanxuan;Yang, Na;Mao, Ruoyu;Hao, Ya;Teng, Da;Wang, Jianhua;Ma, Xuanxuan;Huang, Yinhua;Ma, Xuanxuan;Yang, Na;Mao, Ruoyu;Hao, Ya;Teng, Da;Wang, Jianhua

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关键词： antimicrobial peptide NZ2114; drug delivery system; nanoparticles; PLGA; Staphylococcus epidermidis; biofilms

期刊名称：ANTIBIOTICS-BASEL （ 影响因子：4.8； 五年影响因子：4.9 ）

ISSN： 2079-6382

年卷期： 2024 年 13 卷 3 期

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收录情况： SCI

摘要： Antimicrobial peptides (AMPs) are antibiotic candidates; however, their instability and protease susceptibility limit clinical applications. In this study, the polylactic acid-glycolic acid (PLGA)-polyvinyl alcohol (PVA) drug delivery system was screened by orthogonal design using the double emulsion-solvent evaporation method. NZ2114 nanoparticles (NZ2114-NPs) displayed favorable physicochemical properties with a particle size of 178.11 +/- 5.23 nm, polydispersity index (PDI) of 0.108 +/- 0.10, zeta potential of 4.78 +/- 0.67 mV, actual drug-loading rate of 4.07 +/- 0.37%, encapsulation rate of 81.46 +/- 7.42% and cumulative release rate of 67.75% (120 h) in PBS. The results showed that PLGA encapsulation increased HaCaT cell viability by 20%, peptide retention in 50% serum by 24.12%, and trypsin tolerance by 4.24-fold. Meanwhile, in vitro antimicrobial assays showed that NZ2114-NPs had high inhibitory activity against Staphylococcus epidermidis (S. epidermidis) (4-8 mu g/mL). Colony counting and confocal laser scanning microscopy (CLSM) confirmed that NZ2114-NPs were effective in reducing the biofilm thickness and bacterial population of S. epidermidis G4 with a 99% bactericidal rate of persister bacteria, which was significantly better than that of free NZ2114. In conclusion, the results demonstrated that PLGA nanoparticles can be used as a reliable NZ2114 delivery system for the treatment of biofilm infections caused by S. epidermidis.

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